Bridging environmental mixtures and toxic effects.
Bridging environmental mixtures and toxic effects.
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DOI：
10.1002/etc.2018
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发表时间：
2012-12
影响因子：
4.1
通讯作者：
Anderson, Kim A.
中科院分区：
文献类型：
--
作者：
Allan, Sarah E.;Smith, Brian W.;Tanguay, Robert L.;Anderson, Kim A.
Bridge是一种生物分析工具，它将被动采样与斑马鱼胚胎发育毒性生物测定相结合，为生物可用复杂混合物的毒性提供定量测量。被动采样设备(PSD)从环境中隔离和浓缩生物可用有机污染物，部署在俄勒冈州波特兰市波特兰港超级基金遗址内外的威拉米特河和哥伦比亚河。在2009年夏季和2010年秋季进行了6次抽样活动。用去卷积报告软件分析了PSD提取物中的多环芳烃(PAH)化合物，并筛选出1201种令人关注的化学物质。用斑马鱼胚胎生物测定方法分析了提取物的发育毒性。桥梁提供了关于环境污染物混合物及其毒性的特定地点的、时间可分辨的信息。多变量建模方法被应用于配对的化学和毒性影响数据集，以帮助解开化学和毒性之间的联系。模拟显示了多环芳烃浓度和样品毒性之间的显著相关性，并确定了与观察到的毒性高度相关的多环芳烃分析物的子集。虽然这项研究强调了在复杂混合物中识别特定生物活性化合物的复杂性，但它展示了将毒性效应与环境样品的化学特征联系起来的方法。
BRIDGES is a bioanalytical tool that combines passive sampling with the embryonic zebrafish developmental toxicity bioassay to provide a quantitative measure of the toxicity of bioavailable complex mixtures. Passive sampling devices (PSDs), which sequester and concentrate bioavailable organic contaminants from the environment, were deployed in the Willamette and Columbia Rivers within and outside of the Portland Harbor Superfund site in Portland, Oregon. Six sampling events were conducted in the summer and fall of 2009 and 2010. PSD extracts were analyzed for polycyclic aromatic hydrocarbon (PAH) compounds and screened for 1201 chemicals of concern using deconvolution reporting software. The developmental toxicity of the extracts was analyzed using the embryonic zebrafish bioassay. BRIDGES provided site-specific, temporally resolved information about environmental contaminant mixtures and their toxicity. Multivariate modeling approaches were applied to paired chemical and toxic effects data sets to help unravel chemistry-toxicity associations. Modeling demonstrated a significant correlation between PAH concentrations and the toxicity of the samples and identified a subset of PAH analytes that were the most highly correlated with observed toxicity. Although this research highlights the complexity of discerning specific bioactive compounds in complex mixtures, it demonstrates methods for associating toxic effects with chemical characteristics of environmental samples.
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影响因子：
8.8
作者：
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通讯作者：
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影响因子：
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