A cembranoid from tobacco prevents the expression of nicotine-induced withdrawal behavior in planarian worms.
A cembranoid from tobacco prevents the expression of nicotine-induced withdrawal behavior in planarian worms.
复制标题
DOI：
10.1016/j.ejphar.2009.05.022
复制
发表时间：
2009-08-01
影响因子：
5
通讯作者：
Eterović VA
中科院分区：
文献类型：
--
作者：
Pagán OR;Rowlands AL;Fattore AL;Coudron T;Urban KR;Bidja AH;Eterović VA
使用已发表的行为协议的适应，我们确定，急性暴露于胆碱能化合物尼古丁和氨甲酰胆碱降低的浓度依赖性的方式的Planarian运动。烟草西松烷类化合物（1 S，2 E，4 R，6 R，7 E，11 E）-cembra-2，7，11-triene-4，6-diol（4 R-西松烷类化合物）也降低了真涡虫的运动性。在存在1 μM 4 R-西松烷类化合物的实验中，确实增加了尼古丁诱导的涡虫运动减少的IC 50，但不增加氨甲酰胆碱诱导的减少。当涡虫暴露于尼古丁或氨甲酰胆碱在各自的IC 50值附近的浓度为24小时，然后转移到普通媒体，尼古丁暴露，但不是氨甲酰胆碱或西松烷酸暴露蠕虫显示撤退样的痛苦行为。在实验中，在存在1 μM 4 R-西松烷醇的情况下，将涡虫预先暴露于100 μM尼古丁24 h，戒断样效应显著降低。这些结果表明，4 R-西松烷类化合物可能对烟草滥用研究有价值的应用。这种使用真涡虫的实验方法对于药物滥用和依赖相关化合物的初步筛选是有用的。
Using an adaptation of published behavioral protocols, we determined that acute exposure to the cholinergic compounds nicotine and carbamylcholine decreased planarian motility in a concentration-dependent manner. A tobacco cembranoid (1S,2E,4R,6R,7E,11E)-cembra-2,7,11-triene-4,6-diol (4R-cembranoid), also decreased planarian motility. Experiments in the presence of 1 μM 4R-cembranoid did increase the IC50 for nicotine- but not carbamylcholine-induced decrease in planarian motility. When planarians were exposed for 24 h to either nicotine or carbamylcholine at concentrations near their respective IC50 values and then transferred to plain media, nicotine-exposed, but not carbamylcholine- or cembranoid-exposed worms displayed withdrawal-like distress behaviors. In experiments where planarians were pre-exposed to 100 μM nicotine for 24 h in the presence of 1 μM 4R-cembranoid, the withdrawal-like effects were significantly reduced. These results indicate that the 4R-cembranoid might have valuable applications for tobacco abuse research. This experimental approach using planarians is useful for the initial screening of compounds relevant to drug abuse and dependence.
登录
查看更多内容
影响因子：
10.7
作者：
Nakazawa, M;Cebrià, F;Gojobori, T
通讯作者：
Gojobori, T
DOI：
10.1098/rspb.1979.0081
发表时间：
1979-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DAWKINS, R;KREBS, JR
通讯作者：
KREBS, JR
影响因子：
56.9
作者：
Geffeney, S;Brodie, ED;Brodie, ED
通讯作者：
Brodie, ED
影响因子：
64.8
作者：
Geffeney, SL;Fujimoto, E;Ruben, PC
通讯作者：
Ruben, PC
影响因子：
2.7
作者：
OLSSON, E;HOLTH, A;WAHLBERG, I
通讯作者：
WAHLBERG, I