Atomic Force Microscopy of Side Wall and Septa Peptidoglycan From Bacillus subtilis Reveals an Architectural Remodeling During Growth.
Atomic Force Microscopy of Side Wall and Septa Peptidoglycan From Bacillus subtilis Reveals an Architectural Remodeling During Growth.
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枯草芽孢杆菌侧壁和隔膜肽聚糖的原子力显微镜揭示了生长过程中的结构重塑。
DOI：
10.3389/fmicb.2018.00620
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发表时间：
2018
影响因子：
5.2
通讯作者：
Su HN
中科院分区：
文献类型：
--
作者：
Li K;Yuan XX;Sun HM;Zhao LS;Tang R;Chen ZH;Qin QL;Chen XL;Zhang YZ;Su HN
肽聚糖是细菌细胞壁的基本结构成分。尽管进行了多年的研究，但肽聚糖的结构在很大程度上仍然难以捉摸。在这里，我们报告了来自模式革兰氏阳性细菌枯草芽孢杆菌的肽聚糖的高分辨率结构。我们提供了不同生长阶段肽聚糖结构重塑的高分辨率证据。枯草杆菌AS1.398菌株的侧壁肽聚糖从指数生长期的不规则结构转变为静止相的有序索状结构。侧壁肽聚糖的厚度与生长阶段有关，过渡到稳定期后略有增加。间隔盘逐渐向中心合成，而表面特征不如侧壁成像清晰。与以前的研究相比，我们的结果揭示了不同菌株枯草杆菌肽聚糖的结构略有不同，扩大了我们对枯草杆菌肽聚糖结构特征的认识。
Peptidoglycan is the fundamental structural constituent of the bacterial cell wall. Despite many years of research, the architecture of peptidoglycan is still largely elusive. Here, we report the high-resolution architecture of peptidoglycan from the model Gram-positive bacterium Bacillus subtilis. We provide high-resolution evidence of peptidoglycan architecture remodeling at different growth stages. Side wall peptidoglycan from B. subtilis strain AS1.398 changed from an irregular architecture in exponential growth phase to an ordered cable-like architecture in stationary phase. Thickness of side wall peptidoglycan was found to be related with growth stages, with a slight increase after transition to stationary phase. Septal disks were synthesized progressively toward the center, while the surface features were less clear than those imaged with side walls. Compared with previous studies, our results revealed slight differences in architecture of peptidoglycan from different B. subtilis strains, expanding our knowledge about the architectural features of B. subtilis peptidoglycan.
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影响因子：
3.6
作者：
Turner RD;Vollmer W;Foster SJ
通讯作者：
Foster SJ
影响因子：
3.2
作者：
Dmitriev, BA;Toukach, FV;Ehlers, S
通讯作者：
Ehlers, S
影响因子：
5.2
作者：
de Pedro MA;Cava F
通讯作者：
Cava F
影响因子：
3.6
作者：
Angeles, Danae Morales;Liu, Yun;Scheffers, Dirk-Jan
通讯作者：
Scheffers, Dirk-Jan
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影响因子：
6.4
作者：
Dufrêne YF
通讯作者：
Dufrêne YF