Nanofibrous asymmetric collagen/curcumin membrane containing aspirin-loaded PLGA nanoparticles for guided bone regeneration.
Nanofibrous asymmetric collagen/curcumin membrane containing aspirin-loaded PLGA nanoparticles for guided bone regeneration.
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DOI：
10.1038/s41598-020-75454-2
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发表时间：
2020-10-23
影响因子：
4.6
通讯作者：
Sharifi S
中科院分区：
文献类型：
--
作者：
Ghavimi MA;Bani Shahabadi A;Jarolmasjed S;Memar MY;Maleki Dizaj S;Sharifi S
本研究的目的是开发一种受益于姜黄素和阿司匹林的非对称引导骨再生（GBR）膜。采用静电纺丝技术制备膜，然后通过常规方法进行物理化学表征。用紫外分光光度法测定了阿司匹林的释放曲线。并对膜的抗菌活性进行了评价。我们还评估了所制备的膜对牙髓干细胞（DPSC）的生物相容性和成骨分化的体外影响，并通过组织学方法评估了使用所制备的膜在狗两侧下颌骨缺损中的体内骨再生。表征结果表明，该膜具有纳米纤维单分散性、均匀的网络状形貌、负表面电荷和阿司匹林缓释平台。该膜还对所有测试细菌表现出抗菌作用。姜黄素和阿司匹林的存在下，在不对称的膜增强成骨潜能在转录和翻译水平。动物试验的结果表明，仅28天后，试验区域就完全被新骨填充，而商业膜区域仍然是空的。在测试侧的新骨区域上方也有软组织层。我们认为，在这项工作中制备的膜可以作为GBR膜，以防止软组织占据骨缺损的GBR手术。此外，手术可以受益于这种新型GBR膜的抗菌活性和骨愈合效果，同时促进骨再生。
The goal of the current study was to develop an asymmetric guided bone regeneration (GBR) membrane benefiting from curcumin and aspirin. The membrane was prepared using electrospinning technique and then was physic-chemically characterized by the conventional methods. The release profile of aspirin from the prepared membrane was also measured by ultraviolet spectrophotometry. Also, the antibacterial activities of the membrane was evaluated. We also assessed the in vitro effects of the prepared membrane on the biocompatibility and osteogenic differentiation of dental pulp stem cells (DPSCs), and evaluated in vivo bone regeneration using the prepared membrane in the defects created in both sides of the dog’s jaw by histology. The results from the characterization specified that the membrane was successfully prepared with monodispersed nanosized fibers, uniform network shaped morphology, negative surface charge and sustained release platform for aspirin. The membrane also showed antimicrobial effects against all tested bacteria. The presence of curcumin and aspirin in the asymmetric membrane enhanced osteogenic potential at both transcriptional and translational levels. The results of the animal test showed that the test area was completely filled with new bone after just 28 days, while the commercial membrane area remained empty. There was also a soft tissue layer above the new bone area in the test side. We suggested that the prepared membrane in this work could be used as a GBR membrane to keep soft tissue from occupying bone defects in GBR surgeries. Besides, the surgeries can be benefited from antibacterial activities and bone healing effects of this novel GBR membrane while, simultaneously, promoting bone regeneration.
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影响因子：
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作者：
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DOI：
10.1186/s40199-015-0115-8
发表时间：
2015-06-12
期刊：
Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences
影响因子：
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作者：
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影响因子：
9.7
作者：
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DOI：
10.33945/sami/ecc.2020.1.13
发表时间：
2020-01-01
期刊：
EURASIAN CHEMICAL COMMUNICATION
影响因子：
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作者：
Ghavimi, Mohammad Ali;Negandari, Ramin;Dizaj, Solmaz Maleki
通讯作者：
Dizaj, Solmaz Maleki