How does temperature affect splicing events? Isoform switching of splicing factors regulates splicing of LATE ELONGATED HYPOCOTYL (LHY).
How does temperature affect splicing events? Isoform switching of splicing factors regulates splicing of LATE ELONGATED HYPOCOTYL (LHY).
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DOI：
10.1111/pce.13193
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Nimmo HG
中科院分区：
文献类型：
--
作者：
James AB;Calixto CPG;Tzioutziou NA;Guo W;Zhang R;Simpson CG;Jiang W;Nimmo GA;Brown JWS;Nimmo HG
植物对温度做出反应的方式之一是通过选择性剪接（AS）。先前的工作表明，温度变化影响了几个生物钟基因转录本的剪接。在这里，我们研究了RNA结合剪接因子（SF）在时钟基因晚伸长下胚轴（LHY）的温度敏感性AS中的作用。在野生型植物中，我们从高分辨率温度和时间序列RNA测序实验中表征了SF转录物的温度相关同种型转换和表达模式。此外，我们采用SF突变体植物的定量RT-PCR来探索SF在LHY冷却相关AS中的作用。我们表明，剪接和表达的几个SF响应充分，迅速，和敏感的温度变化，有助于剪接的5′UTR的LHY。此外，在LHY中的剪接位点的选择在一些SF突变体中改变。LHY的5′UTR区域的剪接具有分子恒温器的特征，其中转录物同种型的比率对温度变化敏感至2 °C，并且在宽的动态温度范围内可扩展。我们的工作为SF介导的温度感知与生物钟转录后调控的耦合提供了新的见解。植物对温度的早期反应的机制在很大程度上仍然难以捉摸。在这里，我们研究了RNA结合剪接因子（SF）在时钟基因晚伸长下胚轴的温度敏感性选择性剪接中的作用。我们证明，SF转录本的温度相关亚型转换对温度变化足够迅速和敏感，有助于后期伸长下胚轴5′UTR的剪接。这项工作确立了“剪接因子的剪接”在温度感知与生物钟转录后调控耦合中的作用。
One of the ways in which plants can respond to temperature is via alternative splicing (AS). Previous work showed that temperature changes affected the splicing of several circadian clock gene transcripts. Here, we investigated the role of RNA‐binding splicing factors (SFs) in temperature‐sensitive AS of the clock gene LATE ELONGATED HYPOCOTYL (LHY). We characterized, in wild type plants, temperature‐associated isoform switching and expression patterns for SF transcripts from a high‐resolution temperature and time series RNA‐seq experiment. In addition, we employed quantitative RT‐PCR of SF mutant plants to explore the role of the SFs in cooling‐associated AS of LHY. We show that the splicing and expression of several SFs responds sufficiently, rapidly, and sensitively to temperature changes to contribute to the splicing of the 5′UTR of LHY. Moreover, the choice of splice site in LHY was altered in some SF mutants. The splicing of the 5′UTR region of LHY has characteristics of a molecular thermostat, where the ratio of transcript isoforms is sensitive to temperature changes as modest as 2 °C and is scalable over a wide dynamic range of temperature. Our work provides novel insight into SF‐mediated coupling of the perception of temperature to post‐transcriptional regulation of the clock. The mechanisms involved in the early responses of plants to temperature remain largely elusive. Here, we investigated the role of RNA‐binding splicing factors (SFs) in temperature‐sensitive alternative splicing of the clock gene LATE ELONGATED HYPOCOTYL. We demonstrate that temperature‐associated isoform switching of SF transcripts is sufficiently rapid and sensitive to temperature changes to contribute to the splicing of the 5′UTR of LATE ELONGATED HYPOCOTYL. This work establishes a role for “splicing of splicing factors” in the coupling of the perception of temperature to post‐transcriptional regulation of the clock.
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