Genome-centric investigation of the potential succession pattern in gut microbiota and altered functions under high-protein diet.
Genome-centric investigation of the potential succession pattern in gut microbiota and altered functions under high-protein diet.
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DOI：
10.1016/j.crfs.2023.100600
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发表时间：
2023
影响因子：
6.3
通讯作者：
中科院分区：
文献类型：
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作者：
蛋白质摄入过多被认为是导致肠道微生态失调的一个因素，但高蛋白饮食（HPD）下肠道微生物发生变化的原因和方式尚未完全阐明。本研究采用16S rRNA基因扩增子测序和宏基因组测序技术，对正常饮食组和HPD组小鼠盲肠、结肠和粪便内容物进行分析。然后利用多种统计模型和生物信息学方法对微生物组成和功能的变化进行了深入分析。在这三个生态位中，观察到结肠中的微生物在HPD后表现出最显著的变化，具有较低的α多样性和较高的β多样性。此外，微生物结构的这种变化可能与置换过程和共生群落有关。在这个过程中，大多数物种也在结肠中富集或贫乏。通过分析不同生态位中蛋白质和碳水化合物水解相关的功能基因，我们发现，与富含蛋白质相比，贫碳水化合物提供的碳源可能是推动HPD下结肠中粘蛋白降解物和淀粉酶富集的潜在因素。因此，我们的研究提供了一个新的见解，从微生物功能生态学的角度来理解HPD影响肠道健康的潜在机制。高蛋白饮食主要是通过微生物种类的替换来影响结肠内的微生物群落。该蛋白酶具有由肠道中的各种微生物提供的可替代性。在结肠中观察到由潜在的碳水化合物（碳源）竞争驱动的三种物质替代模式。高蛋白日粮条件下，碳源的相对缺乏促进了粘蛋白降解菌的富集。
Excessive intake of protein has been considered as a factor leading to intestinal microecological disorder, but why and how intestinal microbes change under the high-protein diet (HPD) have yet to be fully elucidated. Here, we performed 16S rRNA gene amplicon sequencing and metagenomic sequencing on contents of cecum, colon and feces from two groups of mice with standard diet (SD) and HPD. And then the microbial alteration of composition and function were deeply analyzed by using several statistical models and bioinformatic methods. Among the three niches, the microbes in the colon are observed to show the most significant change with lower alpha-diversity and higher beta-diversity after HPD. In addition, this alteration of microbial structure may be related to the replacement process and co-occurring community. Most species are also enriched or impoverished in the colon during this process. After analyzing the functional genes related to protein and carbohydrate hydrolysis in different niches, we found that the carbon source provided by poor carbohydrates compared with the rich protein may be the potential factor driving the enrichment of mucin degraders and desulphaters in the colon under HPD. Therefore, our study provided a new insight to understand the underlying mechanism of HPD affecting intestinal health from the perspective of microbial functional ecology. High-protein diet mainly affects the microbial community in the colon by microbial species replacement. The protease has substitutability provided by various microbes in the intestine. Three species replacement patterns driven by potential carbohydrate (carbon source) competition are observed in the colon. The relative scarcity of carbon sources promotes the enrichment of mucin degraders under high-protein diet.
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影响因子：
4.3
作者：
Koga Y
通讯作者：
Koga Y
DOI：
10.1093/database/bas003
发表时间：
2012
期刊：
Database : the journal of biological databases and curation
影响因子：
--
作者：
Eberhardt RY;Haft DH;Punta M;Martin M;O'Donovan C;Bateman A
通讯作者：
Bateman A
影响因子：
10.7
作者：
Huerta-Cepas J;Forslund K;Coelho LP;Szklarczyk D;Jensen LJ;von Mering C;Bork P
通讯作者：
Bork P
DOI：
10.1016/j.bbapap.2015.04.015
发表时间：
2015-08
影响因子：
3.2
作者：
Gerlt, John A.;Bouvier, Jason T.;Davidson, Daniel B.;Imker, Heidi J.;Sadkhin, Boris;Slater, David R.;Whalen, Katie L.
通讯作者：
Whalen, Katie L.
聚糖代谢如何塑造人类肠道微生物群。
DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
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