Cytokine release from alveolar macrophages exposed to ambient particulate matter: heterogeneity in relation to size, city and season.
Cytokine release from alveolar macrophages exposed to ambient particulate matter: heterogeneity in relation to size, city and season.
复制标题
暴露于环境颗粒物问题的肺泡巨噬细胞中的细胞因子释放：与大小，城市和季节有关的异质性。
DOI：
10.1186/1743-8977-2-4
复制
发表时间：
2005-08-17
影响因子：
10
通讯作者：
Schwarze PE
中科院分区：
文献类型：
--
作者：
Hetland RB;Cassee FR;Låg M;Refsnes M;Dybing E;Schwarze PE
几项研究表明，暴露于环境颗粒物（PM）与呼吸道和心血管疾病之间存在关联。炎症似乎在观察到的健康影响中起着重要作用。然而，驱动炎症的主要颗粒组分仍未完全澄清。在这项研究中，代表性的粗（2.5-10 μm）和细（0.1-2.5 μm）颗粒样品从西部，东部，北方和南部欧洲城市（阿姆斯特丹，罗兹，奥斯陆和罗马）在三个季节（春季，夏季和冬季）收集。研究了所有组分在从大鼠肺中分离的原代巨噬细胞中的苦参碱诱导潜力。结果与样品的物理和化学参数相关，以揭示炎症潜力与颗粒的特定特征之间可能的联系。在逐克的基础上进行比较，证明了PM诱导的细胞因子反应的位点特异性和季节性变化。在东部（罗兹）和南部（罗马）城市收集的样本似乎是最有效的。季节性变化最明显的是来自罗兹的样品，春季和夏季样品诱导的最高响应。细胞因子释放的位点特异性或季节性变化不能归因于任何化学参数的变化。来自所有城市的粗级分比相应的细级分更有效地诱导炎性细胞因子白细胞介素-6和肿瘤坏死因子-α。较高水平的特定元素，如铁和铜，一些多环芳烃（PAHs）和内毒素/脂多糖似乎是普遍存在的粗馏分。然而，这些组分含量的变化并不反映由不同粗级分诱导的细胞因子释放的变化。添加多粘菌素B不影响颗粒诱导的细胞因子释放，表明粗级分之间的效价变化不能用内毒素解释。周围PM的炎症潜力表现出异质性与城市和季节。粗颗粒部分始终比相应的细颗粒部分更有效。虽然较高水平的一些元素，PAH和内毒素被发现在粗馏分，特定成分的存在下是不足以解释所有的变化，PM诱导的细胞因子释放。
Several studies have demonstrated an association between exposure to ambient particulate matter (PM) and respiratory and cardiovascular diseases. Inflammation seems to play an important role in the observed health effects. However, the predominant particle component(s) that drives the inflammation is still not fully clarified. In this study representative coarse (2.5–10 μm) and fine (0.1–2.5 μm) particulate samples from a western, an eastern, a northern and a southern European city (Amsterdam, Lodz, Oslo and Rome) were collected during three seasons (spring, summer and winter). All fractions were investigated with respect to cytokine-inducing potential in primary macrophages isolated from rat lung. The results were related to the physical and chemical parameters of the samples in order to disclose possible connections between inflammatory potential and specific characteristics of the particles. Compared on a gram-by gram basis, both site-specific and seasonal variations in the PM-induced cytokine responses were demonstrated. The samples collected in the eastern (Lodz) and southern (Rome) cities appeared to be the most potent. Seasonal variation was most obvious with the samples from Lodz, with the highest responses induced by the spring and summer samples. The site-specific or seasonal variation in cytokine release could not be attributed to variations in any of the chemical parameters. Coarse fractions from all cities were more potent to induce the inflammatory cytokines interleukin-6 and tumour necrosis factor-α than the corresponding fine fractions. Higher levels of specific elements such as iron and copper, some polycyclic aromatic hydrocarbons (PAHs) and endotoxin/lipopolysaccaride seemed to be prevalent in the coarse fractions. However, variations in the content of these components did not reflect the variation in cytokine release induced by the different coarse fractions. Addition of polymyxin B did not affect the particle-induced cytokine release, indicating that the variations in potency among the coarse fractions are not explained by endootoxin. The inflammatory potential of ambient PM demonstrated heterogeneity in relation to city and season. The coarse particle fractions were consistently more potent than the respective fine fractions. Though a higher level of some elements, PAH and endotoxin was found in the coarse fractions, the presence of specific components was not sufficient to explain all variations in PM-induced cytokine release.
登录
查看更多内容
DOI：
10.1165/ajrcmb.25.4.4515
发表时间：
2001-10-01
影响因子：
6.4
作者：
Bonvallot, V;Baeza-Squiban, A;Marano, F
通讯作者：
Marano, F
影响因子：
2.8
作者：
Hetland, RB;Schwarze, PE;Refsnes, M
通讯作者：
Refsnes, M
DOI：
10.1165/rcmb.2003-0300oc
发表时间：
2004-09-01
影响因子：
6.4
作者：
Albrecht, C;Schins, RPF;Borm, PJA
通讯作者：
Borm, PJA
影响因子：
1.7
作者：
Amakawa, K;Terashima, T;Yamaguchi, K
通讯作者：
Yamaguchi, K
DOI：
10.1080/152873902760125741
发表时间：
2002-09-13
影响因子：
2.6
作者：
Huang, SL;Cheng, WL;Chan, CC
通讯作者：
Chan, CC