A methyl jasmonate induced defensin like protein from Panax notoginseng confers resistance against Fusarium solani in transgenic tobacco
A methyl jasmonate induced defensin like protein from Panax notoginseng confers resistance against Fusarium solani in transgenic tobacco
复制标题
三七中茉莉酸甲酯诱导的防御素样蛋白赋予转基因烟草对茄病镰刀菌的抗性
DOI：
10.32615/bp.2019.123
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发表时间：
2019-12
影响因子：
1.5
通讯作者：
Liu DQ
中科院分区：
文献类型：
--
作者：
Wang Q;Qiu BL;Li S;Zhang YP;Cui XM;Ge F;Liu DQ
植物防御素和防御素样蛋白（defensin-like protein，DEFL）是一类富含半胱氨酸的小分子蛋白。它们是植物免疫系统的主要组成部分，参与宿主对生物和非生物胁迫的防御。本研究从中药三七中克隆了一个新的防御素样蛋白（defensin like protein，DEFL）基因PnDEFL 1，并利用逆转录qPCR技术分析了PnDEFL 1在茉莉酸甲酯、水杨酸、乙烯利和H2 O2处理后以及在茄病镰刀菌侵染过程中的表达模式。PnDEFL 1的表达上调表明其对F. Solani感染和所有四种防御相关信号分子。将PnDEFL 1基因与植物表达载体中的绿色荧光蛋白基因融合，转化洋葱表皮细胞。激光扫描共聚焦显微镜证实PnDEFL 1蛋白定位于细胞外区域。此外，在大肠杆菌中表达了PnDEFL 1重组蛋白，并通过亲和层析进行了纯化。对F. solani，F.尖孢霉、葡萄座腔菌和核盘菌。将PnDEFL 1基因转入烟草（Nicotiana tabacum）中，以验证其功能。PnDEFL 1在烟草中的过表达赋予了对F.茄病感染因此，PnDEFL 1基因参与了三七对F.茄病感染
Plant defensins and defensin like protein (DEFL) form a large family of small cysteine-rich proteins. They are major components of plant immune systems, being involved in host defenses against biotic and abiotic stresses. In this study, a novel defensin like protein (DEFL) gene PnDEFL1 was isolated from Panax notoginseng, a traditional Chinese medicinal herb. The expression patterns of PnDEFL1 after treatment with methyl jasmonate, salicylic acid, ethephon, and H2O2, as well as during Fusarium solani infection, were analyzed using reverse transcription qPCR. The up-regulated expression of PnDEFL1 indicated that it responded to F. solani infection and all four defense-related signalling molecules. The PnDEFL1 gene was further fused with the green fluorescent protein gene in a plant expression vector and transformed into onion (Allium cepa) epidermal cells. The laser scanning confocal microscope confirmed that the PnDEFL1 protein localized to the extracellular region. In addition, the recombinant PnDEFL1 protein was expressed in Escherichia coli and purified by affinity chromatography. It had antifungal activities against F. solani, F. oxysporum, Botrosphaeria dothidea, and Sclerotinia sclerotiorum. The PnDEFL1 gene was transferred into tobacco (Nicotiana tabacum) to verify its function. The overexpression of PnDEFL1 in tobacco conferred a high resistance to F. solani infection. Thus, the PnDEFL1 gene is involved in the defense responses of P. notoginseng to F. solani infection.
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影响因子：
2.8
作者：
Gachomo, Emma W.;Jimenez-Lopez, Jose C.;Kotchoni, Simeon O.
通讯作者：
Kotchoni, Simeon O.
影响因子：
--
作者：
Mello Ede O;dos Santos IS;Carvalho Ade O;de Souza LS;de Souza-Filho GA;do Nascimento VV;Machado OL;Zottich U;Gomes VM
通讯作者：
Gomes VM
影响因子：
5.3
作者：
de Beer A;Vivier MA
通讯作者：
Vivier MA
影响因子：
6.9
作者：
W. Broekaert;B. Cammue;M. D. De Bolle;K. Thevissen;G. De Samblanx;R. Osborn;K. Nielson
通讯作者：
W. Broekaert;B. Cammue;M. D. De Bolle;K. Thevissen;G. De Samblanx;R. Osborn;K. Nielson
影响因子：
5.4
作者：
Franco, Octavio L.;Murad, Andre M.;Bloch, Carlos, Jr.
通讯作者：
Bloch, Carlos, Jr.