Effects on g2/m phase cell cycle distribution and aneuploidy formation of exposure to a 60 Hz electromagnetic field in combination with ionizing radiation or hydrogen peroxide in l132 nontumorigenic human lung epithelial cells.
Effects on g2/m phase cell cycle distribution and aneuploidy formation of exposure to a 60 Hz electromagnetic field in combination with ionizing radiation or hydrogen peroxide in l132 nontumorigenic human lung epithelial cells.
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DOI：
10.4196/kjpp.2015.19.2.119
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Lee YS
中科院分区：
文献类型：
--
作者：
Jin H;Yoon HE;Lee JS;Kim JK;Myung SH;Lee YS
本研究的目的是评估暴露于极低频磁场（ELF-MF; 60 Hz，1 mT或2 mT）与应激因素（如电离辐射（IR）或H2 O2）的组合是否会导致非致瘤性人肺上皮L132细胞的基因组不稳定性。为此，检查G2/M期阻滞细胞和非整倍体细胞的百分比。暴露于0.5戈伊IR或0.05 mM H2 O2 9 h导致最高水平的非整倍体;然而，在subG 1期没有观察到细胞，这表明不存在凋亡性细胞死亡。单独暴露于ELF-MF（1 mT或2 mT）不影响G2/M期阻滞细胞、非整倍体细胞或亚G1期细胞群的百分比。此外，当细胞暴露于与IR（0.5戈伊）或H2 O2（0.05 mM）组合的1 mT或2 mT ELF-MF时，ELF-MF没有进一步增加G2/M停滞细胞或非整倍体细胞的百分比。这些结果表明，ELF-MFs单独不诱导G2/M期阻滞或非整倍体，即使当与不同的应激源组合施用时。
The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells were observed in the subG1 phase, which indicated the absence of apoptotic cell death. Exposure to an ELF-MF alone (1 mT or 2 mT) did not affect the percentages of G2/M-arrested cells, aneuploid cells, or the populations of cells in the subG1 phase. Moreover, when cells were exposed to a 1 mT or 2 mT ELF-MF in combination with IR (0.5 Gy) or H2O2 (0.05 mM), the ELF-MF did not further increase the percentages of G2/M-arrested cells or aneuploid cells. These results suggest that ELF-MFs alone do not induce either G2/M arrest or aneuploidy, even when administered in combination with different stressors.
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影响因子：
8
作者：
Huang, L;Snyder, AR;Morgan, WF
通讯作者：
Morgan, WF
DOI：
10.3109/10715769009109662
发表时间：
1990-01-01
期刊：
FREE RADICAL RESEARCH COMMUNICATIONS
影响因子：
--
作者：
GOLDSTEIN, BD;WITZ, G
通讯作者：
WITZ, G
影响因子：
--
作者：
Eleuteri AM;Amici M;Bonfili L;Cecarini V;Cuccioloni M;Grimaldi S;Giuliani L;Angeletti M;Fioretti E
通讯作者：
Fioretti E
DOI：
10.1016/j.mrgentox.2005.03.011
发表时间：
2005-06-06
影响因子：
1.9
作者：
Ivancsits, S;Pilger, A;Rüdiger, HW
通讯作者：
Rüdiger, HW
DOI：
10.1016/0165-1161(90)90041-l
发表时间：
1990-10-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
EASTMOND, DA;PINKEL, D
通讯作者：
PINKEL, D