De Novo Design of Functional Coassembling Organic-Inorganic Hydrogels for Hierarchical Mineralization and Neovascularization.
De Novo Design of Functional Coassembling Organic-Inorganic Hydrogels for Hierarchical Mineralization and Neovascularization.
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DOI：
10.1021/acsnano.0c09814
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发表时间：
2021-07-27
期刊：
影响因子：
17.1
通讯作者：
Mata A
中科院分区：
文献类型：
--
作者：
Okesola BO;Mendoza-Martinez AK;Cidonio G;Derkus B;Boccorh DK;Osuna de la Peña D;Elsharkawy S;Wu Y;Dawson JI;Wark AW;Knani D;Adams DJ;Oreffo ROC;Mata A
由于不同成分的固有性质的独特但互补的性质，结合有机和无机组分的合成纳米结构材料为广泛应用提供了独特的、强大的和通用的材料类别。我们报告了一种基于合成纳米粘土（Laponite，Lap）和肽两亲物（PAs，PAH 3）的超分子体系，该体系被合理地设计成具有高结构完整性和一系列生物活性的纳米结构水凝胶。利用光谱和散射技术和分子动力学模拟方法来确认PAH 3纳米纤维静电吸附并符合Lap纳米盘的表面。电子和原子力显微镜也证实了与拉普共组装后PAH 3纳米纤维的直径和表面积的增加。动态振荡流变学表明，共组装的PAH 3-Lap水凝胶显示出高刚度和强大的自修复行为，而气体吸附分析证实了分层和非均匀的孔隙率。此外，这种独特的结构内的三维（3D）矩阵提供了空间限制的成核和高纵横比的羟基磷灰石纳米棒的分层组织成明确定义的球形簇内的3D矩阵。在体外使用人骨髓源性基质细胞（hBMSC）和离体使用鸡绒毛尿囊膜（CAM）测定来评估有机-无机PAH 3-Lap水凝胶的适用性。结果表明，有机-无机PAH 3-Lap水凝胶促进人骨骼细胞增殖，并且在矿化后与CAM整合，被血管浸润，刺激细胞外基质产生，并且相对于对照促进广泛的矿物质沉积。
Synthetic nanostructured materials incorporating both organic and inorganic components offer a unique, powerful, and versatile class of materials for widespread applications due to the distinct, yet complementary, nature of the intrinsic properties of the different constituents. We report a supramolecular system based on synthetic nanoclay (Laponite, Lap) and peptide amphiphiles (PAs, PAH3) rationally designed to coassemble into nanostructured hydrogels with high structural integrity and a spectrum of bioactivities. Spectroscopic and scattering techniques and molecular dynamic simulation approaches were harnessed to confirm that PAH3 nanofibers electrostatically adsorbed and conformed to the surface of Lap nanodisks. Electron and atomic force microscopies also confirmed an increase in diameter and surface area of PAH3 nanofibers after coassembly with Lap. Dynamic oscillatory rheology revealed that the coassembled PAH3-Lap hydrogels displayed high stiffness and robust self-healing behavior while gas adsorption analysis confirmed a hierarchical and heterogeneous porosity. Furthermore, this distinctive structure within the three-dimensional (3D) matrix provided spatial confinement for the nucleation and hierarchical organization of high-aspect ratio hydroxyapatite nanorods into well-defined spherical clusters within the 3D matrix. Applicability of the organic–inorganic PAH3-Lap hydrogels was assessed in vitro using human bone marrow-derived stromal cells (hBMSCs) and ex vivo using a chick chorioallantoic membrane (CAM) assay. The results demonstrated that the organic–inorganic PAH3-Lap hydrogels promote human skeletal cell proliferation and, upon mineralization, integrate with the CAM, are infiltrated by blood vessels, stimulate extracellular matrix production, and facilitate extensive mineral deposition relative to the controls.
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影响因子：
16.6
作者：
Appel EA;Tibbitt MW;Webber MJ;Mattix BA;Veiseh O;Langer R
通讯作者：
Langer R
影响因子：
4.6
作者：
Kurylo I;Gines G;Rondelez Y;Coffinier Y;Vlandas A
通讯作者：
Vlandas A
影响因子：
9.5
作者：
Cao, Ying;Mei, May Lei;Chu, Chun Hung
通讯作者：
Chu, Chun Hung
影响因子：
17.1
作者：
Brown N;Lei J;Zhan C;Shimon LJW;Adler-Abramovich L;Wei G;Gazit E
通讯作者：
Gazit E
DOI：
10.3390/ma10020119
发表时间：
2017-01-28
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Lemloh ML;Altintoprak K;Wege C;Weiss IM;Rothenstein D
通讯作者：
Rothenstein D