Controlled release of insulin-like growth factor 1 enhances urethral sphincter function and histological structure in the treatment of female stress urinary incontinence in a rat model.
Controlled release of insulin-like growth factor 1 enhances urethral sphincter function and histological structure in the treatment of female stress urinary incontinence in a rat model.
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DOI：
10.1111/bju.13985
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发表时间：
2018-03
影响因子：
4.5
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Yan H;Zhong L;Jiang Y;Yang J;Deng J;Wei S;Opara E;Atala A;Mao X;Damaser MS;Zhang Y
在压力性尿失禁（SUI）大鼠模型中，确定海藻酸盐-聚-L-鸟氨酸-明胶（A-PLO-G）微珠中胰岛素样生长因子1（IGF-1）的控释对尿道外括约肌（EUS）组织再生的影响，因为SUI降低了数百万人的生活质量，特别是阴道分娩的女性，这可能会损伤尿道括约肌。尽管有几种成熟的治疗SUI的方法，生长因子治疗可能提供一种替代方法，以促进尿道括约肌修复。总之，将44只雌性Sprague-Dawley大鼠随机分为4组：阴道扩张（VD），然后尿道周围注射IGF-1-A-PLO-G微珠（VD + IGF-1微珠; 1 × 104微珠/1 mL生理盐水）; VD +空微珠; VD +生理盐水;或假VD+生理盐水（假手术）。尿道功能VD +生理盐水治疗后1周，渗漏点压（LPP）显著降低[平均值（sem）23.9（1.3）cmH 2 O]或VD +空微珠[平均值（sem）21.7（0.8）cmH 2 O）与假手术组相比[平均值（sem）44.4（3.4）cmH 2 O; P < 0.05），表明微珠本身不会在尿道中产生膨胀或阻塞作用。与VD +空微珠相比，VD + IGF-1微珠后1周的LPP显著更高[平均值（sem）28.4（1.2）cmH 2 O]（P < 0.05），并且与假手术大鼠中的LPP没有显著差异，表明甚至在VD后1周就开始了修复作用。组织学分析显示，在VD + IGF-1微珠后1周，EUS中组织良好的骨骼肌纤维和血管发育，相比之下，在VD +盐水或VD +空微珠后1周，大量的肌纤维衰减和解体以及较少的血管形成。IGF-1-A-PLO-G微珠的尿道周围给药通过促进骨骼肌生成和血管再生促进从SUI恢复。这种疗法很有前途，但需要在动物模型和人类中进行详细和长期的研究。
To determine the effects of controlled release of insulin-like growth factor 1 (IGF-1) from alginate-poly-L-ornithine-gelatine (A-PLO-G) microbeads on external urethral sphincter (EUS) tissue regeneration in a rat model of stress urinary incontinence (SUI), as SUI diminishes the quality of life of millions, particularly women who have delivered vaginally, which can injure the urethral sphincter. Despite several well-established treatments for SUI, growth factor therapy might provide an alternative to promote urethral sphincter repair. In all, 44 female Sprague-Dawley rats were randomised into four groups: vaginal distension (VD) followed by periurethral injection of IGF-1-A-PLO-G microbeads (VD + IGF-1 microbeads; 1 × 104 microbeads/1 mL normal saline); VD + empty microbeads; VD + saline; or sham-VD + saline (sham). Urethral function (leak-point pressure, LPP) was significantly lesser 1 week after VD + saline [mean (sem) 23.9 (1.3) cmH2O] or VD + empty microbeads [mean (sem) 21.7 (0.8) cmH2O) compared to the sham group [mean (sem) 44.4 (3.4) cmH2O; P < 0.05), indicating that the microbeads themselves do not create a bulking or obstructive effect in the urethra. The LPP was significantly higher 1 week after VD + IGF-1 microbeads [mean (sem) 28.4 (1.2) cmH2O] compared to VD + empty microbeads (P < 0.05), and was not significantly different from the LPP in sham rats, demonstrating an initiation of a reparative effect even at 1 week after VD. Histological analysis showed well-organised skeletal muscle fibres and vascular development in the EUS at 1 week after VD + IGF-1 microbeads, compared to substantial muscle fibre attenuation and disorganisation, and less vascular formation at 1 week after VD + saline or VD + empty microbeads. Periurethral administration of IGF-1-A-PLO-G microbeads facilitates recovery from SUI by promoting skeletal myogenesis and revascularisation. This therapy is promising, but detailed and longer term studies in animal models and humans are needed.
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影响因子：
3.4
作者：
Apel, Peter J.;Ma, Jianjun;Callahan, Michael;Northam, Casey N.;Alton, Timothy B.;Sonntag, William E.;Li, Zhongyu
通讯作者：
Li, Zhongyu
影响因子：
2
作者：
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通讯作者：
DeLancey, John O. L.
影响因子：
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作者：
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通讯作者：
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影响因子：
14
作者：
Impellitteri, Nicholas A.;Toepke, Michael W.;Levengood, Sheeny K. Lan;Murphy, William L.
通讯作者：
Murphy, William L.
影响因子：
2
作者：
Jiang, Hai-Hong;Pan, Hui Q.;Gustilo-Ashby, Marcus A.;Gill, Bradley;Glaab, Jonathan;Zaszczurynski, Paul;Damaser, Margot
通讯作者：
Damaser, Margot