Comparative analysis of alternative splicing, alternative polyadenylation and the expression of the two KIN genes from cytoplasmic male sterility cabbage (Brassica oleracea L. var. capitata L.)
Comparative analysis of alternative splicing, alternative polyadenylation and the expression of the two KIN genes from cytoplasmic male sterility cabbage (Brassica oleracea L. var. capitata L.)
复制标题
细胞质雄性不育甘蓝（Brassica oleracea L. var. capitata L.）两种KIN基因的选择性剪接、选择性多腺苷酸化和表达的比较分析
DOI：
10.1007/s00438-014-0815-7
复制
发表时间：
2014-02
影响因子：
3.1
通讯作者：
Xinmin Zhong
中科院分区：
文献类型：
--
作者：
Wuhong Wang;Zhichen Yue;Juanli Lei;Xinmin Zhong
KIN基因是冷调控基因家族的重要成员。它们在植物许多器官的发育过程中发挥着独特的作用，并对各种非生物胁迫作出反应。然而，关于KIN基因在甘蓝细胞质雄性不育（Brassica oleracea L. var. capitata L.）中的表达调控尚不清楚。我们进行了全基因组分析，鉴定了CMS白菜的KIN基因。鉴定出两个非冗余的KIN基因，分别命名为BoKIN1 （Bol021262）和BoKIN2 （Bol030498）。逆转录酶PCR检测到BoKIN1（4个AS产物）和BoKIN2（3个AS产物）的备选剪接产物。此外，在CMS白菜中观察到BoKIN1和BoKIN2的选择性聚腺苷化（APA），导致其转录本中出现可变的3 ' utr。此外，我们还分析了BoKIN1-0和BoKIN2-0的内含子在非生物胁迫下的不同器官和幼嫩叶片中的转录水平，其中BoKIN1-0和BoKIN2-0的内含子被完全拼接。我们的数据表明，BoKIN1-0在各种器官中都有高表达，而BoKIN2-0只在雄蕊中表达。我们的研究还表明，BoKIN1-0在暴露于脱落酸处理和冷、热胁迫下的植物幼叶中显著上调。BoKIN1和BoKIN2在pre-mRNA加工过程中具有不同的AS和APA模式，并且在表达模式和转录水平上存在差异。BoKIN1广泛参与器官发育并响应多种非生物胁迫，而BoKIN2在CMS白菜的雄蕊发育中起主要作用。
The KIN genes are crucial members of the cold-regulated gene family. They play exclusive roles during the developmental processes of many organs and respond to various abiotic stresses in plants. However, little is known about the regulation of KIN gene expression in cytoplasmic male sterility (CMS) cabbages (Brassica oleracea L. var. capitata L.). We carried out a genome-wide analysis to identify the KIN genes in the CMS cabbage. Two non-redundant KIN genes, named BoKIN1 (Bol021262) and BoKIN2 (Bol030498), were identified. Reverse transcriptase PCR detected alternative splicing (AS) products of BoKIN1 (four AS products) and BoKIN2 (three AS products). In addition, alternative polyadenylation (APA) was observed for BoKIN1 and BoKIN2 in the CMS cabbage, resulting in variable 3′UTRs in their transcripts. Furthermore, the transcription levels of BoKIN1-0 and BoKIN2-0, the introns of which were spliced completely, were analyzed in various organs and young leaves treated by abiotic stresses. Our data indicated that BoKIN1-0 is highly expressed in various organs, whereas BoKIN2-0 is expressed exclusively in the stamen. Our study also suggested that BoKIN1-0 was upregulated significantly in young leaves of plants exposed to abscisic acid treatment, and cold and heat stress. BoKIN1 and BoKIN2 had differential AS and APA patterns in pre-mRNA processing, and showed differences in their expression patterns and transcript levels. BoKIN1 participates widely in organ development and responds to diverse abiotic stresses, whereas BoKIN2 plays a main role in stamen development in the CMS cabbage.
登录
查看更多内容
Bolbase：甘蓝综合基因组数据库
影响因子：
4.4
作者：
Yu J;Zhao M;Wang X;Tong C;Huang S;Tehrim S;Liu Y;Hua W;Liu S
通讯作者：
Liu S
影响因子：
7.4
作者：
W. Orr;B. Iu;T. White;L. Robert;Jas Singh
通讯作者：
W. Orr;B. Iu;T. White;L. Robert;Jas Singh
影响因子：
7.7
作者：
Shinozaki, K;YamaguchiShinozaki, K
通讯作者：
YamaguchiShinozaki, K
影响因子：
7.4
作者：
BOOTHE, JG;DEBEUS, MD;JOHNSONFLANAGAN, AM
通讯作者：
JOHNSONFLANAGAN, AM
影响因子：
5.1
作者：
Nakashima, K;Fujita, Y;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K