Wolbachia endosymbionts manipulate the self-renewal and differentiation of germline stem cells to reinforce fertility of their fruit fly host.
Wolbachia endosymbionts manipulate the self-renewal and differentiation of germline stem cells to reinforce fertility of their fruit fly host.
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DOI：
10.1371/journal.pbio.3002335
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发表时间：
2023-10
期刊：
影响因子：
9.8
通讯作者：
中科院分区：
文献类型：
--
作者：
α变形杆菌Wolbachia pipientis感染世界各地的节肢动物和线虫物种，使其成为宿主生物控制的关键目标。沃尔巴克氏体驱动的宿主生殖操作，如细胞质不相容性（CI），被认为是这些细胞内细菌在宿主种群中高频率出现的原因。积极的，也许是互惠的，生殖操作也会增加感染频率，但还没有得到很好的理解。在这里，我们确定的分子和细胞的机制，沃尔巴克氏体影响生殖干细胞（GSC）的自我更新和分化的分子不同的过程。我们证明，wMel感染挽救了缺乏翻译调节因子mei-P26的果蝇的生育能力，并且足以无限期地维持不育的纯合mei-P26敲低的种群。细胞学显示，wMel通过恢复适当的pMad、Bam、Sxl和Orb表达来减轻mei-P26损失的影响。在具有野生型生育力的俄勒冈州R文件中，wMel感染提高了终生卵孵化率。通过真核和细菌转录物的双RNAseq定量探索这些表型揭示了wMel感染在mRNA水平上挽救和抵消了由mei-P26损失诱导的许多基因表达变化。总体而言，我们表明，wMel感染有益地加强了宿主的生育能力在mRNA，蛋白质和表型水平，这些机制可能会促进互利共生的出现和主机生殖操作的故障。沃尔巴克氏体细菌共生体被利用作为生物控制剂，利用其操纵宿主生育力。这项研究表明，wMel菌株可以对突变型和野生型果蝇的果蝇发育产生有益的影响;这种表型对于长期使用这些细菌进行宿主种群控制至关重要。
The alphaproteobacterium Wolbachia pipientis infects arthropod and nematode species worldwide, making it a key target for host biological control. Wolbachia-driven host reproductive manipulations, such as cytoplasmic incompatibility (CI), are credited for catapulting these intracellular bacteria to high frequencies in host populations. Positive, perhaps mutualistic, reproductive manipulations also increase infection frequencies, but are not well understood. Here, we identify molecular and cellular mechanisms by which Wolbachia influences the molecularly distinct processes of germline stem cell (GSC) self-renewal and differentiation. We demonstrate that wMel infection rescues the fertility of flies lacking the translational regulator mei-P26 and is sufficient to sustain infertile homozygous mei-P26-knockdown stocks indefinitely. Cytology revealed that wMel mitigates the impact of mei-P26 loss through restoring proper pMad, Bam, Sxl, and Orb expression. In Oregon R files with wild-type fertility, wMel infection elevates lifetime egg hatch rates. Exploring these phenotypes through dual-RNAseq quantification of eukaryotic and bacterial transcripts revealed that wMel infection rescues and offsets many gene expression changes induced by mei-P26 loss at the mRNA level. Overall, we show that wMel infection beneficially reinforces host fertility at mRNA, protein, and phenotypic levels, and these mechanisms may promote the emergence of mutualism and the breakdown of host reproductive manipulations. Wolbachia bacterial symbionts are being harnessed as biological control agents by exploiting their manipulation of host fertility. This study reveals that the wMel strain can have a beneficial impact on fruit fly development in both mutant and wild-type flies; this phenotype is essential to the long-term use of these bacteria for host population control.
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影响因子：
4.4
作者：
Casper-Lindley, Catharina;Kimura, Scott;Sullivan, William
通讯作者：
Sullivan, William
释放不兼容的男性在澳大利亚的野生和沃尔巴契亚岛伊德斯埃及埃及埃及的人群中进行了强烈的抑制。
DOI：
10.1073/pnas.2106828118
发表时间：
2021-10-12
影响因子：
11.1
作者：
Beebe NW;Pagendam D;Trewin BJ;Boomer A;Bradford M;Ford A;Liddington C;Bondarenco A;De Barro PJ;Gilchrist J;Paton C;Staunton KM;Johnson B;Maynard AJ;Devine GJ;Hugo LE;Rasic G;Cook H;Massaro P;Snoad N;Crawford JE;White BJ;Xi Z;Ritchie SA
通讯作者：
Ritchie SA
影响因子：
3.7
作者：
Bean DM;Heimbach J;Ficorella L;Micklem G;Oliver SG;Favrin G
通讯作者：
Favrin G
影响因子：
10.5
作者：
BOPP, D;BELL, LR;SCHEDL, P
通讯作者：
SCHEDL, P
影响因子：
6.7
作者：
Ferree PM;Frydman HM;Li JM;Cao J;Wieschaus E;Sullivan W
通讯作者：
Sullivan W