A stilbene synthase allele from a Chinese wild grapevine confers resistance to powdery mildew by recruiting salicylic acid signalling for efficient defence.
A stilbene synthase allele from a Chinese wild grapevine confers resistance to powdery mildew by recruiting salicylic acid signalling for efficient defence.
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DOI：
10.1093/jxb/erw351
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发表时间：
2016-10
影响因子：
6.9
通讯作者：
Nick P
中科院分区：
文献类型：
--
作者：
Jiao Y;Xu W;Duan D;Wang Y;Nick P
中国野生葡萄对白粉病的抗性不同于基于二苯乙烯合成酶启动子将水杨酸引入植物抗真菌蛋白合成的效应器触发免疫。二苯乙烯类化合物是葡萄属植物的中枢保卫素，二苯乙烯合成酶(STS)的诱导是葡萄抗病的关键。在这里，我们通过与来自葡萄品种的同源基因的比较，探讨了利用从中国野生葡萄假网纹葡萄(VpSTS)分离的STS等位基因进行抗病育种的可能性。《Carigane》(VvSTS)。虽然两个等位基因的编码区非常相似(在氨基酸水平上有99%的同源性)，但启动子区域明显不同。通过异源系统在拟南芥中的表达，我们发现野生中国葡萄的等位基因可以促进二苯乙烯类化合物的积累和对白粉病的抗性，而栽培葡萄的等位基因不能。为了剖析驱动该启动子激活的上游信号，我们在葡萄细胞培养中使用了双荧光素酶报告系统。我们显示从野生葡萄启动子对水杨酸(SA)和病原体相关分子模式(PAMP)flg22的反应性增强，茉莉酸(JA)对这两个等位基因的诱导相等，而对细胞死亡诱导激发子Harpin缺乏反应。VpSTS启动子的这种高SA反应依赖于钙内流、RboH的氧化爆发、丝裂原激活的蛋白激酶(MAPK)信号和JA合成。我们在一个模型的背景下整合了这些数据，在这个模型中，假网纹病菌的抗性与更有效地招募SA信号来合成植保素有关。
The resistance of the Chinese wild grapevine Vitis pseudoreticulata to powdery mildew is shown to differ from effector-triggered immunity by recruiting salicylic acid into phytoalexin synthesis based on a specific stilbene synthase promoter. Stilbenes are central phytoalexins in Vitis, and induction of the key enzyme stilbene synthase (STS) is pivotal for disease resistance. Here, we address the potential for breeding resistance using an STS allele isolated from Chinese wild grapevine Vitis pseudoreticulata (VpSTS) by comparison with its homologue from Vitis vinifera cv. ‘Carigane’ (VvSTS). Although the coding regions of both alleles are very similar (>99% identity on the amino acid level), the promoter regions are significantly different. By expression in Arabidopsis as a heterologous system, we show that the allele from the wild Chinese grapevine can confer accumulation of stilbenes and resistance against the powdery mildew Golovinomyces cichoracearum, whereas the allele from the vinifera cultivar cannot. To dissect the upstream signalling driving the activation of this promoter, we used a dual-luciferase reporter system in a grapevine cell culture. We show elevated responsiveness of the promoter from the wild grape to salicylic acid (SA) and to the pathogen-associated molecular pattern (PAMP) flg22, equal induction of both alleles by jasmonic acid (JA), and a lack of response to the cell death-inducing elicitor Harpin. This elevated SA response of the VpSTS promoter depends on calcium influx, oxidative burst by RboH, mitogen-activated protein kinase (MAPK) signalling, and JA synthesis. We integrate the data in the context of a model where the resistance of V. pseudoreticulata is linked to a more efficient recruitment of SA signalling for phytoalexin synthesis.
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影响因子：
6.9
作者：
Duan D;Halter D;Baltenweck R;Tisch C;Tröster V;Kortekamp A;Hugueney P;Nick P
通讯作者：
Nick P
影响因子：
6.9
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作者：
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通讯作者：
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影响因子：
6.9
作者：
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通讯作者：
Nick P