Genetic diversity of stilbene metabolism in Vitis sylvestris.
Genetic diversity of stilbene metabolism in Vitis sylvestris.
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DOI：
10.1093/jxb/erv137
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发表时间：
2015-06
影响因子：
6.9
通讯作者：
Nick P
中科院分区：
文献类型：
--
作者：
Duan D;Halter D;Baltenweck R;Tisch C;Tröster V;Kortekamp A;Hugueney P;Nick P
我们表明，祖先的栽培葡萄藤窝藏遗传因素，增加二苯乙烯类的诱导性与增加抗性的重要病原体葡萄藤霜霉病。芪类化合物是葡萄藤重要的次生代谢产物，是植物抗毒素的中心成分，是基础免疫的重要组成部分。本研究对葡萄属欧亚种的潜在遗传变异进行了研究。在此基础上，对栽培葡萄藤的祖先-欧洲赤霞珠进行了研究，以确定其芪类化合物的产量和在抗病育种中的潜在用途。在V. vinifera ssp.森林植物。显示了在响应UV-C脉冲中诱导的二苯乙烯的丰度和分布的基因型差异。出现了两个芪类化合物的“化学变体”簇：一个簇表现出芪类化合物的快速和强烈的积累，几乎完全以非糖基化白藜芦醇和葡萄苷的形式，而第二个簇积累了较少的芪类化合物和相对较高比例的白藜芦醇和糖基化白藜芦醇苷。对于所有86种基因型，观察到的芪模式的时间依赖性：白藜芦醇苷，白藜芦醇，和piceatannol积累较早，而葡萄糖苷被发现后。进一步观察到，在芪积累的基因型差异之前，查尔酮合酶（CHS）和芪相关基因的转录本的差异积累：苯丙氨酸铵裂解酶（PAL），芪合酶（StSy），和白藜芦醇合酶（RS）。筛选的人口对霜霉病的葡萄（霜霉病葡萄）的易感性显示了相当大的变化。与低二苯乙烯基因型相比，高二苯乙烯诱导的基因型亚群的敏感性显著降低，并且对于代表性基因型，可以表明UV对二苯乙烯合酶的诱导与病原菌的诱导相关。
We show that the ancestor of cultivated grapevine harbours genetic factors that increase the inducibility of stilbenes correlated with increased resistance to the important pathogen grapevine downy mildew. Stilbenes, as important secondary metabolites of grapevine, represent central phytoalexins and therefore constitute an important element of basal immunity. In this study, potential genetic variation in Vitis vinifera ssp. sylvestris, the ancestor of cultivated grapevine, was sought with respect to their output of stilbenes and potential use for resistance breeding. Considerable variation in stilbene inducibility was identified in V. vinifera ssp. sylvestris. Genotypic differences in abundance and profiles of stilbenes that are induced in response to a UV-C pulse are shown. Two clusters of stilbene ‘chemovars’ emerged: one cluster showed quick and strong accumulation of stilbenes, almost exclusively in the form of non-glycosylated resveratrol and viniferin, while the second cluster accumulated fewer stilbenes and relatively high proportions of piceatannol and the glycosylated piceid. For all 86 genotypes, a time dependence of the stilbene pattern was observed: piceid, resveratrol, and piceatannol accumulated earlier, whereas the viniferins were found later. It was further observed that the genotypic differences in stilbene accumulation were preceded by differential accumulation of the transcripts for chalcone synthase (CHS) and stilbene-related genes: phenylalanine ammonium lyase (PAL), stilbene synthase (StSy), and resveratrol synthase (RS). A screen of the population with respect to susceptibility to downy mildew of grapevine (Plasmopara viticola) revealed considerable variability. The subpopulation of genotypes with high stilbene inducibility was significantly less susceptible as compared with low-stilbene genotypes, and for representative genotypes it could be shown that the inducibility of stilbene synthase by UV correlated with the inducibility by the pathogen.
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影响因子：
6.5
作者：
Kortekamp, A
通讯作者：
Kortekamp, A
影响因子：
3.8
作者：
Celimene, CC;Smith, DR;Stanosz, GR
通讯作者：
Stanosz, GR
影响因子：
3.7
作者：
Chang X;Heene E;Qiao F;Nick P
通讯作者：
Nick P
影响因子：
4.4
作者：
Polesani M;Desario F;Ferrarini A;Zamboni A;Pezzotti M;Kortekamp A;Polverari A
通讯作者：
Polverari A
影响因子：
2.9
作者：
Kodan, A;Kuroda, H;Sakai, F
通讯作者：
Sakai, F