Transcriptional activity of the FUT1 gene promoter region in pigs.
Transcriptional activity of the FUT1 gene promoter region in pigs.
复制标题
猪 FUT1 基因启动子区的转录活性
DOI：
10.3390/ijms141224126
复制
发表时间：
2013-12-11
影响因子：
5.6
通讯作者：
Bao W
中科院分区：
文献类型：
--
作者：
Zi C;Wu Z;Wang J;Huo Y;Zhu G;Wu S;Bao W
本研究旨在通过对猪α-L，2-岩藻糖基转移酶基因启动子区域转录活性的分析，为猪FUT1基因的调控机制提供理论依据。根据已获得的启动子序列，分别用限制性内切酶KpnI和HindIII设计启动子上游和下游的引物，通过插入部分缺失的启动子序列构建了pGL3-启动子的重组载体。瞬时转染HEK293细胞观察突变情况，荧光素酶活性检测启动子区域的转录活性。FUT1基因5‘侧翼区(−1150~+50bp)具有启动子活性。−1150bp~−849bp区域对该基因有负调控作用。重组表达载体pGL3-898的荧光素酶活性最强，随着缺失区域的增加，荧光素酶活性呈下降趋势。成功构建和验证了FUT1基因的正负调控区和核心启动子区域，为深入研究FUT1基因的转录调控机制提供了新的思路。
This study aims to provide a theoretical basis on the regulatory mechanism of the α-l,2-fucosyltransferase (FUT1) gene in pigs by analyzing the transcriptional activity of its promoter region. On the basis of the previously obtained promoter sequence, primers upstream and downstream of the gene were designed using the restriction endonucleases KpnI and HindIII respectively, and the recombinant plasmids of the pGL3-promoter were constructed by inserting promoter sequences with partially missing regions. The resultant mutants were observed by transient transfection assay into HEK293 cells, and the transcriptional activity of the promoter region was determined by luciferase activity. The 5′-flanking region of the FUT1 gene (−1150 to +50 bp) exhibited promoter activity. The −1150-bp to −849-bp region showed negative regulation of the gene. The recombinant plasmid pGL3-898 showed the strongest luciferase activity, and the activity showed a decreasing trend when the deleted region was increased. Recombinant plasmids were successfully constructed, verified, and the positive and negative regulation areas and core promoter region were detected, providing a deeper insight into the transcriptional regulatory mechanism of the FUT1 gene.
登录
查看更多内容
影响因子：
2.9
作者：
Jha, Abhimanyu Kumar;Nikbakht, Mohsen;Kaur, Jagdeep
通讯作者：
Kaur, Jagdeep
影响因子：
2.8
作者：
Ren, Zhuqing;Liu, Wanghong;Xiong, Yuanzhu
通讯作者：
Xiong, Yuanzhu
亚洲野猪和中西方商品猪品种α-1-岩藻糖基转移酶（FUT1）基因的遗传变异
影响因子：
2.6
作者：
Bao, W. B.;Wu, S. L.;Chen, G. H.
通讯作者：
Chen, G. H.
DOI：
10.1164/ajrccm.160.3.9812024
发表时间：
1999-09-01
影响因子：
24.7
作者：
Burchard, EG;Silverman, EK;Drazen, JM
通讯作者：
Drazen, JM
影响因子：
3.2
作者：
Meijerink, E;Neuenschwander, S;Vögeli, P
通讯作者：
Vögeli, P