Role for glial cells in regulating the functional expression of neuropeptide Y (NPY) neurons in aggregate cultures derived from dissociated fetal brain cells.
Role for glial cells in regulating the functional expression of neuropeptide Y (NPY) neurons in aggregate cultures derived from dissociated fetal brain cells.
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神经胶质细胞在调节来自分离胎儿脑细胞的聚集培养物中神经肽 Y (NPY) 神经元功能表达中的作用。
DOI：
10.1002/jnr.490380412
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发表时间：
1994
影响因子：
4.2
通讯作者：
Lu,G
中科院分区：
文献类型：
--
作者：
Barnea,A;Cho,G;Lu,G
本实验室的一系列研究建立了表达神经肽Y（NPY）的胎鼠脑细胞的聚集培养系统，该系统可作为研究神经胶质细胞-神经元旁分泌相互作用在发育性神经肽Y神经元表达中的作用的模型。在该系统中，NPY的产生随着培养时间的推移而逐渐增加，并且由毛喉素（FOR）和佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）诱导。我们解决了以下问题：是否在没有神经胶质细胞（特别是星形胶质细胞）的情况下，NPY神经元的功能表达受损，如果是这样的话，由脑细胞（完整的聚集体）组成的聚集体的分泌产物可以恢复受损的NPY神经元的功能？从17日龄胎鼠皮质生成聚集体，并在无血清培养基中维持13-15天。阿糖胞苷（CA;剂量为0.5-8 μM）在第1天加入培养物中，并在第15天通过测量3 H胸苷掺入DNA和通过星形胶质细胞标记物胶质细胞酸性蛋白（GFAP）的免疫染色来验证消除胶质细胞的有效性。将第13-15天的基础NPY产生和FOR（10 μM）+ PMA（20 nM）刺激的NPY产生作为功能标准。FOR + PMA诱导对照培养物（无CA）中NPY产量增加102倍。CA以剂量依赖性方式抑制基础和FOR + PMA诱导的NPY产生和DNA合成：在6 μM CA下，基础NPY产生减少约50%，FOR + PMA刺激的NPY产生和DNA合成完全受到抑制，星形胶质细胞基本被消除。竞争剂量100 μM脱氧胞苷完全逆转6 μM CA对DNA合成和基础及FOR + PMA刺激的NPY产生的影响。来自完整聚集体的条件培养基完全恢复了CA处理的聚集体中的基础和部分（约50%）恢复的FOR + PMA刺激的NPY产生。这些结果牵连神经胶质细胞（星形胶质细胞？）和/或神经胶质-神经元旁分泌相互作用调节培养物中NPY神经元的发育表达，使得cAMP和蛋白激酶C途径的激活导致NPY产生增加。© 1994 Wiley利斯公司
A series of studies from our laboratory have established an aggregate culture system of fetal rat brain cells expressing neuropeptide Y (NPY) which can serve as a model to study the role of glia‐neuron paracrine interactions in the developmental expression of NPY neurons. In this system, NPY production increases progressively with culture‐age and it is induced by forskolin (FOR) and phorbol 12‐myristate 13‐acetate (PMA). We addressed the following question: Is the functional expression of the NPY neurons impaired in the absence of glial cells (particularly astrocytes) and if so, can secretory products of aggregates composed of the full complement of brain cells ( intact aggregates) restore the function of the impaired NPY neurons? Aggregates were generated from 17‐day‐old fetal rat cortex and maintained in serum‐free medium for 13–15 days. Cytosine arabinoside (CA; doses of 0.5–8 μM) was added to the cultures on day 1 and the effectiveness in elimination of glial cells was verified on day 15 by measuring the incorporation of3H thymidine into DNA and by immunostaining for the astrocyte marker glial fibrillary acidic protein (GFAP). Basal NPY production and FOR (10 μM) + PMA (20 nM) stimulated production of NPY on days 13–15 were taken as functional criteria. FOR + PMA induced ≈2‐fold increase in NPY production in control cultures (no CA). CA inhibited both basal and FOR + PMA induced production of NPY and DNA synthesis in a dose‐dependent manner: at 6 μM CA, basal NPY production was reduced by about 50%, FOR + PMA stimulated production of NPY and DNA synthesis were completely inhibited, and astrocytes were essentially eliminated. A competing dose of 100 μM deoxycytidine completely reversed the effects of 6 μM CA on DNA synthesis and basal and FOR + PMA stimulated production of NPY. Conditioned media derived from intact aggregates completely restored basal and partially (≈50%) restored FOR + PMA stimulated production of NPY in CA‐treated aggregates. These results implicate secretory products of glial cells (astrocytes?) and/or glial‐neuron paracrine interactions in regulating the developmental expression of NPY neurons in culture such that activation of the cAMP and protein kinase C pathways leads to increased production of NPY. © 1994 Wiley‐Liss, Inc.
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影响因子：
8.7
作者：
FATHMAN, CG;KIMOTO, M
通讯作者：
KIMOTO, M
影响因子：
15.3
作者：
J. McNicholas;D. Murphy;L. Matis;R. Schwartz;E. Lerner;C. Janeway;Patricia P. Jones
通讯作者：
Patricia P. Jones
影响因子：
15.3
作者：
L. Matis;P. Jones;D. Murphy;SM Hedrick;E. Lerner;C. Janeway;J. McNicholas;R. Schwartz
通讯作者：
R. Schwartz
用同种反应性克隆 T 细胞系检测 F1 杂交小鼠中 Ia 糖蛋白复合物的差异表达。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Conrad,PJ;Lerner,EA;Murphy,DB;Jones,PP;JanewayJr,CA
通讯作者：
JanewayJr,CA
DOI：
10.1073/pnas.80.24.7621
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MENGLEGAW, L;MCDEVITT, HO
通讯作者：
MCDEVITT, HO