Laser-fabricated cell patterning stencil for single cell analysis.
Laser-fabricated cell patterning stencil for single cell analysis.
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DOI:
10.1186/s12896-017-0408-8
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发表时间:
2017-12-19
影响因子:
3.5
通讯作者:
Meldrum DR
中科院分区:
文献类型:
--
作者:
Messner JJ;Glenn HL;Meldrum DR
Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.
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