Laser-fabricated cell patterning stencil for single cell analysis.

Laser-fabricated cell patterning stencil for single cell analysis.
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DOI:
10.1186/s12896-017-0408-8
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发表时间:
2017-12-19
期刊:
影响因子:
3.5
通讯作者:
Meldrum DR
Meldrum DR
中科院分区:
工程技术3区
文献类型:
--
作者:
Messner JJ;Glenn HL;Meldrum DR

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哺乳动物细胞的精确空间定位和分离是许多单细胞实验方法和生物工程应用的关键组成部分。尽管已经证明了多种细胞图案化方法,但这些方法中的许多使细胞经受高应力环境,歧视某些表型,或者是实施的挑战。在这里,我们展示了一种快速,简单,不加选择,和最小干扰的细胞图案化方法,使用激光制造的聚合物模板。模板制造过程中不需要模板基板对准,并很容易适应各种基板的几何形状和实验。
Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.
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