Characterization of peripheral blood mononuclear cells gene expression profiles of pediatric Staphylococcus aureus persistent and non-carriers using a targeted assay.
Characterization of peripheral blood mononuclear cells gene expression profiles of pediatric Staphylococcus aureus persistent and non-carriers using a targeted assay.
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DOI：
10.1016/j.micinf.2020.07.006
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发表时间：
2020-11
影响因子：
5.8
通讯作者：
Brown EL
中科院分区：
文献类型：
--
作者：
Israelsson E;Chaussabel D;Fischer RSB;Moore HC;Robinson DA;Dunkle JW;Essigmann HT;Record S;Brown EL
先天免疫缺陷影响许多不同的生理系统，一些对原发性免疫缺陷疾病患者的研究表明，先天免疫系统成分在疾病预防或细菌病原体定植中的重要性。为了评估先天免疫系统对金黄色葡萄球菌鼻腔定植的作用，通过分析共聚集基因集（模块），对儿童金黄色葡萄球菌鼻腔持续携带者（n=14）和非携带者（n=15）的先天免疫反应进行了分析。我们用1)一组TLR配体刺激这些受试者先前冷冻的外周血单个核细胞（PBMCs）， 2)活的金黄色葡萄球菌（菌株的混合物或各自载体分离物的刺激），或3)热杀死的金黄色葡萄球菌。当TLR配体刺激pbmc时，我们发现载体和非载体之间的反应没有差异。然而，PBMC基因表达谱在持久性和非持久性之间存在差异。活的或死的金黄色葡萄球菌刺激后的金黄色葡萄球菌携带者。这些观察结果表明，对金黄色葡萄球菌持续携带易感的个体可能具有活/死细菌识别途径的差异，并且金黄色葡萄球菌持续携带者和非金黄色葡萄球菌携带者之间的先天途径信号传导不同。
Defects in innate immunity affect many different physiologic systems and several studies of patients with primary immunodeficiency disorders demonstrated the importance of innate immune system components in disease prevention or colonization of bacterial pathogens. To assess the role of the innate immune system on nasal colonization with Staphylococcus aureus, innate immune responses in pediatric S. aureus nasal persistent carriers (n=14) and non-carriers (n=15) were profiled by analyzing co-clustered gene sets (modules). We stimulated previously frozen peripheral blood mononuclear cells (PBMCs) from these subjects with i) a panel of TLR ligands, ii) live S. aureus (either a mixture of strains or stimulation with respective carriage isolates), or iii) heat-killed S. aureus. We found no difference in responses between carriers and non-carriers when PBMCs were stimulated with a panel of TLR ligands. However, PBMC gene expression profiles differed between persistent and non-S. aureus carriers following stimulation with either live or dead S. aureus. These observations suggest that individuals susceptible to persistent carriage with S. aureus may possess differences in their live/dead bacteria recognition pathway and that innate pathway signaling is different between persistent and non-carriers of S. aureus.
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影响因子：
30.5
作者：
Alsina, Laia;Israelsson, Elisabeth;Altman, Matthew C.;Dang, Kristen K.;Ghandil, Pegah;Israel, Laura;von Bernuth, Horst;Baldwin, Nicole;Qin, Huanying;Jin, Zongbo;Banchereau, Romain;Anguiano, Esperanza;Ionan, Alexei;Abel, Laurent;Puel, Anne;Picard, Capucine;Pascual, Virginia;Casanova, Jean Laurent;Chaussabel, Damien
通讯作者：
Chaussabel, Damien
影响因子：
3.7
作者：
Brown EL;Below JE;Fischer RS;Essigmann HT;Hu H;Huff C;Robinson DA;Petty LE;Aguilar D;Bell GI;Hanis CL
通讯作者：
Hanis CL
影响因子：
4.6
作者：
Molteni M;Gemma S;Rossetti C
通讯作者：
Rossetti C
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
100.3
作者：
Blander, J. Magarian;Sander, Leif E.
通讯作者：
Sander, Leif E.