Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection.
Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection.
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DOI：
10.1016/j.exppara.2012.03.005
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发表时间：
2012-06
影响因子：
2.1
通讯作者：
Fairhurst, Rick M.
中科院分区：
文献类型：
--
作者：
Tokumasu, Fuyuki;Ostera, Graciela R.;Amaratunga, Chanaki;Fairhurst, Rick M.
ζ电位（ZP）是细胞表面的电化学性质，其由暴露在细胞膜表面的分子的净电荷确定。膜蛋白对细胞表面的总净电荷有贡献，并且可以通过其拷贝数的变化和分子间相互作用的变化来改变ZP。恶性疟原虫通过在细胞表面放置“旋钮”样结构来广泛重塑其宿主红细胞（RBC）膜。使用电泳迁移率测定，我们发现人类红细胞的平均ZP为-15.7 mV。在感染恶性疟原虫滋养体的红细胞（“iRBC”）中，平均ZP显著降低（-14.6 mV，p<0.001）。通过神经氨酸酶处理从细胞表面去除唾液酸显著降低了RBC（-6.06 mV）和iRBC（-4.64 mV）的ZP。寄生虫诱导的ZP变化因恶性疟原虫克隆和iRBC表面上的结节的存在而异。ZP值的变化伴随着iRBC与人微血管内皮细胞（MVEC）结合的改变。这些数据表明，寄生虫衍生的结蛋白有助于iRBC的ZP，并且iRBC和MVEC膜之间的静电和疏水相互作用参与细胞粘附。
The zeta potential (ZP) is an electrochemical property of cell surfaces that is determined by the net electrical charge of molecules exposed at the surface of cell membranes. Membrane proteins contribute to the total net electrical charge of cell surfaces and can alter ZP through variation in their copy number and changes in their intermolecular interactions. Plasmodium falciparum extensively remodels its host red blood cell (RBC) membrane by placing ‘knob’-like structures at the cell surface. Using an electrophoretic mobility assay, we found that the mean ZP of human RBCs was −15.7 mV. In RBCs infected with P. falciparum trophozoites (‘iRBCs’), the mean ZP was significantly lower (−14.6 mV, p<0.001). Removal of sialic acid from the cell surface by neuraminidase treatment significantly decreased the ZP of both RBCs (−6.06 mV) and iRBCs (−4.64 mV). Parasite-induced changes in ZP varied by P. falciparum clone and the presence of knobs on the iRBC surface. Variations in ZP values were accompanied by altered binding of iRBCs to human microvascular endothelial cells (MVECs). These data suggest that parasite-derived knob proteins contribute to the ZP of iRBCs, and that electrostatic and hydrophobic interactions between iRBC and MVEC membranes are involved in cytoadherence.
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影响因子：
3.8
作者：
Jan, K M;Chien, S
通讯作者：
Chien, S
影响因子：
3.2
作者：
Haldar, Kasturi;Mohandas, Narla
通讯作者：
Mohandas, Narla
DOI：
10.1046/j.1365-2818.2003.01219.x
发表时间：
2003-09-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
Tokumasu, F;Dvorak, J
通讯作者：
Dvorak, J
影响因子：
20.3
作者：
Murphy, SC;Samuel, BU;Haldar, K
通讯作者：
Haldar, K
影响因子：
2.1
作者：
Aikawa, M;Kamanura, K;Tandler, B
通讯作者：
Tandler, B