Antioxidant neoagarooligosaccharides (NAOs) and dietary fiber production from red algae Gracilariopsis lemaneiformis using enzyme assisted one-step process
Antioxidant neoagarooligosaccharides (NAOs) and dietary fiber production from red algae Gracilariopsis lemaneiformis using enzyme assisted one-step process
复制标题
使用酶辅助一步法从红藻龙须菜中生产抗氧化新琼脂低聚糖 (NAO) 和膳食纤维
DOI：
10.1016/j.foodhyd.2021.107382
复制
发表时间：
2021-11
影响因子：
10.7
通讯作者：
Jiafu Lin
中科院分区：
文献类型：
--
作者：
Tao Song;Ling Liu;Qianlin Tang;Shengwei Xiang;Binkai Wang;Shuzhen Zhang;Xinrong Wang;Yiwen Chu;Dan Luo;Jiafu Lin
红藻是水产养殖中最广泛种植的大型藻类作物，它们通常直接在日常饮食中摄入，并用作食品水胶体提取的原料。目前红藻开发的困境是其附加值低。在这里，我们提出了一个酶辅助一步法（ESOP），将Gracilariopsis lemaneiformis均质化，以高价值的新琼胶寡糖（NAO）和膳食纤维的同时。首先，我们优化了水解工艺（使用以下条件：15 g wetG. lemaneiformis/150 mL缓冲液，15 min均质化和25 U负载酶量），产生17.13 mg粗NAO。其次，通过TLC、CNMR和MALDI-TOF-MS分析表明，NAO可以被多种取代基修饰。在DPPH、ABTS和自由基清除实验中，NAO在不同浓度下均表现出一定的抗氧化活性。第三，制备了膳食纤维浓缩物，其膳食纤维含量为60.78%，蛋白质含量为4.19%，水分含量为8.43%，灰分含量为2.06%。膳食纤维浓缩物在食品工业中可能具有巨大的潜力，因为其生理特性，包括其在30-1150 °C的热稳定性，持水力（24.20 g/g）和持油量（8.26 g/g）。因此，G.采用员工持股计划，首次将马粪鱼同时转化为高附加值的非食用油和膳食纤维。该工艺对环境友好，有效成分得到了保留，为进一步综合利用其他海藻或含凝胶状多糖的植物提供了线索。
Red algae are the most widely cultivated macroalgae crops in aquaculture, and they are often directly ingested in a daily diet and used as raw material for food hydrocolloid extraction. The current dilemma regarding red algae exploitation involves their low added value. Here, we propose using an enzyme-assisted one-step process (ESOP) to convert homogenizedGracilariopsis lemaneiformisto high valued neoagarooligosaccharides (NAOs) and dietary fibre simultaneously. First, we optimized the hydrolysis process (the following conditions were used: 15 g wetG. lemaneiformis/150 mL buffer, 15 min homogenization and 25 U load enzyme amount) to produce 17.13 mg crude NAOs. Second, TLC, CNMR and MALDI-TOF-MS were performed, and the results indicated that NAOs may be modified by a variety of substitutions. Meanwhile, NAOs showed antioxidant activities in the DPPH, ABTS and free radical scavenging experiments at different concentrations. Third, dietary fibre concentrates were prepared and shown to have 60.78% dietary fibre, 4.19% protein, 8.43% moisture and 2.06% ash contents. Dietary fibre concentrate might have great potential in the food industry because of its physiological properties, including its thermostability at 30–1150 °C, water holding capacity (24.20 g/g) and oil holding capacity (8.26 g/g). Therefore,G. lemaneiformiswas firstly converted into high value-added NAOs and dietary fibre at the same time using ESOP. This process was environmentally friendly and preserved the active compounds, and therefore provided clues for the further comprehensive use of other seaweeds or plants containing gel-like polysaccharides.
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影响因子：
4.1
作者：
FLEURY, N;LAHAYE, M
通讯作者：
LAHAYE, M
DOI：
10.1016/bs.abr.2019.11.008
发表时间：
2020
期刊：
--
影响因子：
--
作者：
Hugo Pliego-Cortés;I. Wijesekara;Marie Lang;N. Bourgougnon;G. Bedoux
通讯作者：
Hugo Pliego-Cortés;I. Wijesekara;Marie Lang;N. Bourgougnon;G. Bedoux
影响因子：
5.8
作者：
Maeda, H;Yamamoto, R;Tochikubo, O
通讯作者：
Tochikubo, O
影响因子：
3.1
作者：
DUCKWORT.M;YAPHE, W
通讯作者：
YAPHE, W
影响因子：
11.2
作者：
Melo, MRS;Feitosa, JPA;de Paula, RCM
通讯作者：
de Paula, RCM