In vitro maturation: a five‐year experience
In vitro maturation: a five‐year experience
复制标题
体外成熟：五年经验
DOI：
10.1111/j.1600-0412.2011.01299.x
复制
发表时间：
2012
影响因子：
4.3
通讯作者：
Strowitzki T
中科院分区：
文献类型：
--
作者：
Roesner S;von Wolff M;Eberhardt I;Beuter-Winkler P;Toth B;Strowitzki T
目的：尽管一些患者可能会从减少促卵泡激素（FSH）的应用中受益，但体外成熟（IVM）属于辅助生殖的罕见治疗选择。我们总结了我们五年的IVM经验。设计。回顾性，观察性研究。设置。生殖医学，大学医院。样本：2005年2月至2009年12月115例多囊卵巢综合征（PCOS）患者以及卵巢过度刺激综合征（OHSS）患者。刺激开始于月经周期的第3-10天，FSH剂量为125 IU/天，持续3天。在注射FSH的第三天或之后一天诱导排卵，并在33-38小时后进行取卵。卵母细胞在IVM培养基中培养24小时。取卵后一天进行受精，两天后进行胚胎移植。主要结果指标：妊娠率。115例患者入选，215例卵母细胞回收（卵胞浆内单精子注射：n=125，59%;体外受精：n=73，34.4%），177例胚胎移植。IVM的主要原因是PCOS（71.7%）和OHSS（15.0%）。平均取卵数为8.9个，其中5.9个（64%）成熟，2.8个（45.1%）受精，2.1个移植。每次移植的妊娠率为15.3%（n=27），其中13例活产（7.3%），1例宫内死亡（0.6%），4例流产（2.3%）和9例生化妊娠（5.1%）。在61例中，受精卵母细胞冷冻和32冷冻移植进行，导致三个pregnancy.Conclusions.虽然妊娠率低，IVM是非常方便的患者，由于低FSH剂量和低成本的预约。
Objective.Although some patients may benefit from reduced follicle‐stimulating hormone (FSH) application, in‐vitro maturation (IVM) belongs to the rare treatment options in assisted reproduction. We summarize our five‐year IVM experience.Design.Retrospective, observational study.Setting.Reproductive Medicine, University Hospital. Sample.115 patients with polycystic ovary syndrome (PCOS) as well as patients after ovarian hyperstimulation syndrome (OHSS) from February 2005 to December 2009.Material and Methods. Stimulation started between day 3–10 of the menstrual cycle and FSH dosage was 125IU/day over three days. Ovulation was induced on the third day of FSH injection or one day afterwards and oocyte retrieval was performed 33–38 hours later. Oocytes were cultivated for 24 hours in IVM medium. Fertilization was carried out one day after oocyte retrieval and embryo transfer two days afterwards.Main outcome measures.Pregnancy rates.Results. 115 patients were included and 215 oocyte retrievals (intracytoplasmic sperm injection:n=125, 59%; in vitro fertilization:n=73, 34.4%) with 177 embryo transfers performed. The main reasons for IVM were: PCOS (71.7%) and OHSS (15.0%). Mean number of oocytes was 8.9/oocyte retrieval with 5.9 (64%) becoming mature, 2.8 (45.1%) being fertilized and 2.1 transferred. Pregnancy rate per transfer was 15.3% (n=27) with 13 live births (7.3%), one intrauterine death (0.6%), four miscarriages (2.3%) and nine biochemical pregnancies (5.1%). In 61 cases, fertilized oocytes were frozen and 32 cryotransfers were performed, resulting in three pregnancies.Conclusions.Although the pregnancy rate was low, IVM is very convenient for patients due to low FSH dosages and few appointments at low cost.
登录
查看更多内容
来自未刺激的多囊卵巢的卵母细胞的体外成熟。
影响因子：
4
作者：
Seang Tan;T. Child
通讯作者：
T. Child
人类胚胎再植入后的出生[信件]
DOI：
--
发表时间：
1978
期刊：
影响因子：
--
作者：
Steptoe Pc;Edwards Rg
通讯作者：
Edwards Rg
接受未成熟卵母细胞体外成熟治疗的排卵和无排卵女性的 FSH 和雌二醇基础血清水平。
影响因子：
6.1
作者：
T. Child;C. Sylvestre;I. Pirwany;Seang Tan
通讯作者：
Seang Tan
影响因子：
6.1
作者：
Lin, YH;Hwang, JL;Chen, PH
通讯作者：
Chen, PH
影响因子：
6.1
作者：
Söderström-Anttila, V;Mäkinen, S;Suikkari, AM
通讯作者：
Suikkari, AM