Cystine supplementation sustains plasma mercaptalbumin levels in rats fed low-protein diets more effectively than methionine.
Cystine supplementation sustains plasma mercaptalbumin levels in rats fed low-protein diets more effectively than methionine.
复制标题
补充胱氨酸比蛋氨酸更有效地维持低蛋白饮食大鼠血浆巯基白蛋白水平。
DOI：
10.3164/jcbn.20-146
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发表时间：
2021-09
影响因子：
2.4
通讯作者：
Kuwahata M
中科院分区：
文献类型：
--
作者：
Yano Y;Maeda C;Kaneko I;Kobayashi Y;Aoi W;Kuwahata M
我们最近报道，饮食胱氨酸维持低蛋白饮食大鼠血浆巯基白蛋白水平。本研究旨在比较补充胱氨酸和蛋氨酸（另一种含硫氨基酸）的低蛋白饮食对大鼠血浆巯基白蛋白水平的影响。雄性Sprague-Dawley大鼠喂食20%大豆蛋白分离物饮食（对照组）、5%大豆蛋白分离物饮食（低蛋白组）或补充有甲硫氨酸（低蛋白+Met组）或胱氨酸（低蛋白+Cyss组）的5%大豆蛋白分离物饮食1周。 低蛋白+Met组巯基白蛋白占血浆总白蛋白的百分比显著低于对照组和低蛋白+Cyss组。 低蛋白+Met组和低蛋白+Cys组血细胞中肿瘤坏死因子-α、白细胞介素-6、白细胞介素-1 β和环氧合酶2的mRNA水平无显著差异。 治疗与丁硫氨酸-（S，R）-亚砜亚胺，谷胱甘肽合成的抑制剂，没有影响的百分比巯基白蛋白内总血浆白蛋白在大鼠喂养的低蛋白饮食补充胱氨酸。这些结果表明，补充胱氨酸可能比蛋氨酸更有效地维持血浆巯基白蛋白水平的蛋白质营养不良大鼠。胱氨酸可能通过谷胱甘肽非依赖性途径调节血浆巯基白蛋白水平。
We recently reported that dietary cystine maintained plasma mercaptalbumin levels in rats fed low-protein diets. The present study aimed to compare the influence of low-protein diets supplemented with cystine and methionine, which is another sulfur amino acid, on plasma mercaptalbumin levels in rats. Male Sprague–Dawley rats were fed a 20% soy protein isolate diet (control group), 5% soy protein isolate diet (low-protein group) or 5% soy protein isolate diet supplemented with either methionine (low-protein + Met group) or cystine (low-protein + Cyss group) for 1 week. The percentage of mercaptalbumin within total plasma albumin of the low-protein + Met group was significantly lower than that of the control and low-protein + Cyss groups. No significant differences in the mRNA levels of tumor necrosis factor-α, interleukin-6, interleukin-1β, and cyclooxygenase 2 in blood cells were observed between the low-protein + Met and low-protein + Cyss groups. Treatment with buthionine-(S,R)-sulfoximine, an inhibitor of glutathione synthesis, did not influence the percentage of mercaptalbumin within total plasma albumin in rats fed the low-protein diet supplemented with cystine. These results suggest that supplementation with cystine may be more effective than that with methionine to maintain plasma mercaptalbumin levels in rats with protein malnutrition. Cystine might regulate plasma mercaptalbumin levels via the glutathione-independent pathway.
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影响因子：
7
作者：
Bourgonje, Arno R.;Gabriels, Ruben Y.;Dijkstra, Gerard
通讯作者：
Dijkstra, Gerard
影响因子：
1.6
作者：
Okawa, Hiroshi;Morita, Tatsuya;Sugiyama, Kimio
通讯作者：
Sugiyama, Kimio
影响因子：
5
作者：
DOUMAS, BT;WATSON, WA;BIGGS, HG
通讯作者：
BIGGS, HG
DOI：
10.1016/j.bbagen.2009.05.018
发表时间：
2009-10-01
影响因子：
3
作者：
Kim, C. J.;Kovacs-Nolan, J.;Mine, Y.
通讯作者：
Mine, Y.
影响因子：
9.8
作者：
Fukuhara, Saki;Yasukawa, Keiko;Inoguchi, Toyoshi
通讯作者：
Inoguchi, Toyoshi