Genetic and cellular evidence of decreased inflammation associated with reduced incidence of posttraumatic arthritis in MRL/MpJ mice.
Genetic and cellular evidence of decreased inflammation associated with reduced incidence of posttraumatic arthritis in MRL/MpJ mice.
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DOI：
10.1002/art.37796
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发表时间：
2013-03
影响因子：
--
通讯作者：
Olson, Steven A.
中科院分区：
文献类型：
--
作者：
Lewis, John S., Jr.;Furman, Bridgette D.;Zeitler, Evan;Huebner, Janet L.;Kraus, Virginia B.;Guilak, Farshid;Olson, Steven A.
在小鼠关节内骨折模型中检查炎症与创伤后关节炎之间的关系。雄性C57 BL/6和MRL/MpJ“超级愈合者”小鼠使用先前建立的方法接受胫骨平台骨折。在骨折后0（4小时内）、1、3、5、7、28和56天处死小鼠。在骨折前和骨折后0、1、3、5和7天采集滑膜组织样品，使用RT-PCR检测促炎细胞因子的基因表达。收集滑液和血清样品以使用ELISA测量细胞因子浓度。组织学分析用于评价整个关节滑膜炎和软骨退化，免疫组织化学用于评价所有时间点关节组织中白细胞介素-1的分布。与C57 BL/6小鼠相比，MRL/MpJ小鼠关节损伤后关节内和全身炎症较低，表现为滑膜组织中TNF-α和IL-1β的基因表达较低，以及滑液、血清和关节组织中IL-1α和IL-1β的蛋白水平较低。此外，MRL/MpJ小鼠滑膜组织中巨噬细胞炎性蛋白（MIP）和巨噬细胞衍生趋化因子（MDC/CCL 22）的基因表达较低，关节损伤后滑膜巨噬细胞的急性和晚期浸润减少。C57 BL/6小鼠表现出比MRL/MpJ小鼠更高的炎症水平，MRL/MpJ小鼠在该模型中受到保护免于创伤后关节炎。因此，这些数据表明关节组织炎症与小鼠创伤后关节炎之间存在关联。
To examine the relationship between inflammation and post-traumatic arthritis in a murine intra-articular fracture model. Male C57BL/6 and MRL/MpJ “superhealer” mice received tibial plateau fractures using a previously established method. Mice were sacrificed at 0 (within 4 hours), 1, 3, 5, 7, 28 and 56 days after fracture. Synovial tissue samples were taken prior to fracture and at 0, 1, 3, 5 and 7 days to examine gene expression of pro-inflammatory cytokines using RT-PCR. Synovial fluid and serum samples were collected to measure cytokine concentrations using ELISA. Histologic analysis was used to evaluate whole joint synovitis and cartilage degradation, and immunohistochemistry to evaluate the distribution of interleukin-1 in the joint tissues from all time points. Compared to the C57BL/6 mice, the MRL/MpJ mice had lower intra-articular and systemic inflammation following joint injury, as evidenced by lower gene expression of TNF-α and IL-1β in synovial tissue, and lower protein levels of IL-1α and IL-1β in the synovial fluid, serum, and joint tissues. Furthermore, MRL/MpJ mice had lower gene expression of macrophage inflammatory proteins (MIPs) and macrophage derived chemokine (MDC/CCL22) in synovial tissue, and reduced acute and late-stage infiltration of synovial macrophages after joint injury. C57BL/6 mice exhibited higher levels of inflammation than MRL/MpJ mice, which are protected from post-traumatic arthritis in this model. These data thus suggest an association between joint tissue inflammation and post-traumatic arthritis in mice.
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滑膜巨噬细胞和巨噬细胞产生的细胞因子在驱动聚集蛋白聚糖酶、基质金属蛋白酶和骨关节炎中其他破坏性和炎症反应中的作用。
影响因子：
4.9
作者：
Bondeson J;Wainwright SD;Lauder S;Amos N;Hughes CE
通讯作者：
Hughes CE
DOI：
10.1016/s0304-4165(00)00118-5
发表时间：
2000-12-15
影响因子：
3
作者：
Li, X;Mohan, S;Baylink, DJ
通讯作者：
Baylink, DJ
影响因子：
5
作者：
Goldring, M B
通讯作者：
Goldring, M B
影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
影响因子：
3.8
作者：
Flytlie, Helene Aarslev;Hvid, Malene;Deleuran, Bent
通讯作者：
Deleuran, Bent