A 15-base-pair element activates the SPS4 gene midway through sporulation in Saccharomyces cerevisiae
A 15-base-pair element activates the SPS4 gene midway through sporulation in Saccharomyces cerevisiae
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15 个碱基对元件在酿酒酵母孢子形成过程中激活 SPS4 基因
DOI：
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发表时间：
1995
影响因子：
5.3
通讯作者：
J. Segall
中科院分区：
文献类型：
--
作者：
S. Hepworth;L. K. Ebisuzaki;J. Segall
酿酒酵母的孢子形成代表了一个简单的发育过程，其中减数分裂和孢子壁形成的事件伴随着时间上不同类别的基因的顺序激活。在这项研究中，我们研究了SPS 4基因的表达，该基因属于一组在孢子形成中途被激活的基因。我们通过监测5 '-侧翼序列的连续缺失对引入MATa/MAT α δ sps 4/δ sps 4菌株的质粒携带型SPS 4表达的影响，绘制了SPS 4调控区的上游边界。该分析表明，调控区的5'边界在该基因的推定TATA盒的50 bp内。通过测试跨越该边界的各种寡核苷酸和下游序列激活异源启动子表达的能力，我们发现15-bp序列足以充当孢子形成特异性上游激活序列。这个15 bp的片段，命名为UASSPS 4，激活表达的CYC 1-lacZ报告基因中途通过孢子形成，并在两个方向上同样活跃。将UAS片段延伸到包括相邻的14-bp使其活性增强10倍。我们发现，SPS 4的表达受到调节的方式不同的早期减数分裂基因：突变的UME 6没有导致SPS 4的营养表达，和孢子化特异性表达延迟突变的IME 2。体内和体外试验表明，营养细胞中存在的一种因子与UASSPS 4元件结合。我们推测，在孢子形成过程中，该因子被修饰为SPS 4基因的激活剂，或者，它招募启动子的激活剂。
Sporulation of the yeast Saccharomyces cerevisiae represents a simple developmental process in which the events of meiosis and spore wall formation are accompanied by the sequential activation of temporally distinct classes of genes. In this study, we have examined expression of the SPS4 gene, which belongs to a group of genes that is activated midway through sporulation. We mapped the upstream boundary of the regulatory region of SPS4 by monitoring the effect of sequential deletions of 5'-flanking sequence on expression of plasmid-borne versions of SPS4 introduced into a MATa/MAT alpha delta sps4/delta sps4 strain. This analysis indicated that the 5' boundary of the regulatory region was within 50 bp of the putative TATA box of the gene. By testing various oligonucleotides that spanned this boundary and the downstream sequence for their ability to activate expression of a heterologous promoter, we found that a 15-bp sequence sufficed to act as a sporulation-specific upstream activation sequence. This 15-bp fragment, designated UASSPS4, activated expression of a CYC1-lacZ reporter gene midway through sporulation and was equally active in both orientations. Extending the UAS fragment to include the adjacent 14-bp enhanced its activity 10-fold. We show that expression of SPS4 is regulated in a manner distinct from that of early meiotic genes: mutation of UME6 did not lead to vegetative expression of SPS4, and sporulation-specific expression was delayed by mutation of IME2. In vivo and in vitro assays suggested that a factor present in vegetative cells bind to the UASSPS4 element. We speculate that during sporulation this factor is modified to serve as an activator of the SPS4 gene or, alternatively, that it recruits an activator to the promoter.
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酵母 UME6 基因产物是 CAR1 URS1 阻遏物结合位点介导的转录抑制所必需的。
影响因子：
14.9
作者：
Park,HD;Luche,RM;Cooper,TG
通讯作者：
Cooper,TG
酵母 IME1 基因产物转录激活的遗传证据。
影响因子：
3.3
作者：
Smith,HE;Driscoll,SE;Sia,RA;Yuan,HE;Mitchell,AP
通讯作者：
Mitchell,AP
DOI：
10.1073/pnas.81.24.7860
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GUARENTE, L;HOAR, E
通讯作者：
HOAR, E
影响因子：
10.5
作者：
Randy Strich;Richard T. Surosky;Camille M. Steber;Evelyne Dubois;F. Messenguy;Rochelle Easton Esposito
通讯作者：
Randy Strich;Richard T. Surosky;Camille M. Steber;Evelyne Dubois;F. Messenguy;Rochelle Easton Esposito
普遍存在的上游抑制序列控制酵母中诱导型精氨酸酶基因的激活。
DOI：
10.1073/pnas.84.12.3997
发表时间：
1987
影响因子：
11.1
作者：
Sumrada,RA;Cooper,TG
通讯作者：
Cooper,TG