Phospholipid composition of postmortem schizophrenic brain by 31P NMR spectroscopy.
Phospholipid composition of postmortem schizophrenic brain by 31P NMR spectroscopy.
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DOI：
10.1002/mrm.21820
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发表时间：
2009-01
影响因子：
3.3
通讯作者：
Mrak, Robert E.
中科院分区：
文献类型：
--
作者：
Pearce, John M.;Komoroski, Richard A.;Mrak, Robert E.
由于磷脂（PL）组成和代谢的变化引起的细胞膜异常与精神分裂症的发病机制有关。这项工作通常评估了来自非神经组织如红细胞和血小板的膜磷脂。高分辨率31 P NMR光谱被用来表征20例精神分裂症患者，20例对照组和7例其他精神疾病患者（精神病对照组）死后大脑灰质的PL。从额叶，颞叶和枕叶皮质的组织中提取与己烷-异丙醇，和31 P NMR光谱中获得的有机溶剂系统，以解决主要PL类（基于头基）和子类（基于在sn-1位置的链接）。令人惊讶的是，重复测量的多变量方差分析显示，各组之间没有总体差异。三组之间任何单个PL亚类（包括溶血磷脂）均无显著差异（p<0.05）。所有磷脂酰乙醇胺头基的总和显着低于精神分裂症患者比对照组或精神病控制额叶皮层。当前结果与这些相同样品中PL代谢物水溶液的先前结果相关性最低。精神分裂症患者体内31 P MRS测量的代谢物变化似乎不能反映PL浓度的变化。目前的结果提供了很少的支持磷脂假说的精神分裂症。
Cell membrane abnormalities due to changes in phospholipid (PL) composition and metabolism have been implicated in schizophrenia pathogenesis. That work has generally assessed membrane phospholipids from non-neural tissues such as erythrocytes and platelets. High resolution 31P NMR spectroscopy was used to characterize PLs of gray matter in postmortem brain for 20 schizophrenics, 20 controls, and 7 patients with other mental illnesses (psychiatric controls). Tissues from frontal, temporal, and occipital cortices were extracted with hexane-isopropanol, and 31P NMR spectra were obtained in an organic-solvent system to resolve the major PL classes (based on headgroups) and subclasses (based on linkage at the sn-1 position). Surprisingly, repeated-measures multivariate analysis of variance revealed no overall differences among the groups. There were no significant differences (p<0.05) among the three groups for any individual PL subclass, including lysophospholipids. The sum of all phosphatidylethanolamine headgroups was significantly lower for schizophrenics than for controls or psychiatric controls in frontal cortex. The present results are minimally correlated with previous results for aqueous PL metabolites on these same samples. The metabolite changes measured by in vivo 31P MRS in schizophrenia do not appear to reflect PL concentration changes. The present results offer very little support for the phospholipid hypothesis of schizophrenia.
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影响因子：
4.5
作者：
MAHADIK, SP;MUKHERJEE, S;SCHEFFER, R
通讯作者：
SCHEFFER, R
影响因子：
3.3
作者：
PEARCE, JM;KOMOROSKI, RA
通讯作者：
KOMOROSKI, RA
DOI：
10.1016/0952-3278(92)90058-q
发表时间：
1992-05-01
影响因子：
3
作者：
DEMISCH, L;HEINZ, K;KIRSTEN, R
通讯作者：
KIRSTEN, R
影响因子：
2.3
作者：
Komoroski, RA;Pearce, JM;Karson, CN
通讯作者：
Karson, CN
影响因子：
--
作者：
FEIGHNER, JP;WOODRUFF, RA;GUZE, SB
通讯作者：
GUZE, SB