Slow-growing pollen-tube of colchicine-induced 2n pollen responsible for low triploid production rate in Populus
Slow-growing pollen-tube of colchicine-induced 2n pollen responsible for low triploid production rate in Populus
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秋水仙碱诱导的 2n 花粉花粉管生长缓慢是杨树三倍体生产率低的原因
DOI：
10.1007/s10681-017-1881-9
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发表时间：
2017-03
期刊：
影响因子：
1.9
通讯作者：
张平冬
中科院分区：
文献类型：
--
作者：
张平冬
为揭示毛白杨无性系三倍体产生率低的机理，对毛白杨无性系5088进行了减数分裂观察、秋水仙素诱导2n花粉、秋水仙素诱导2n花粉杂交和花粉萌发试验。培养24 h后开始减数分裂。结果表明，减数分裂是一个连续的、异步的过程。用GLM单变量分析秋水仙素诱导的2n花粉的出现率，发现在减数分裂的优势时期之间存在显著差异（F = 45.822，P = 0.000）。秋水仙素注射时间的差异有统计学意义（F = 10.150，P = 0.004）。秋水仙素诱导2n花粉的最有效时期发生在偶线期至终变期之间。在3346个后代中，通过流式细胞术分析和染色体计数确认了68个三倍体。最高的三倍体诱导率为13.2%，显著低于预期的三倍体产生率47.0%，表明秋水仙素诱导的2n花粉在受精过程中是弱的。秋水仙素诱导2n花粉的频率与三倍体产生率呈正相关（r = 0.961，P = 0.001），说明三倍体产生率随秋水仙素诱导2n花粉频率的增加而增加。用GLM单变量分析表明，花粉类型（F = 87.50，P 0.001）和花粉萌发时间（F = 7.61，P = 0.002）对花粉管长度有显著影响。单倍体花粉管显著长于秋水仙素诱导的2n花粉管。这表明单倍体花粉在受精过程中具有优势。
To reveal the mechanism of low triploid production, meiotic observation, induction of 2n pollen by colchicine, hybridization of colchicine-induced 2n pollen and pollen germination were conducted in Populus tomentosa clone 5088. Meiosis of the PMCs was initiated 24 h after it was cultured in the greenhouse. Results showed that the meiosis developed in a consecutive, asynchronous process. Analysis of the occurrence rate of colchicine-induced 2n pollen using the GLM-univariate revealed significant differences among the dominant meiotic stages (F = 45.822, P = 0.000). Significant differences also occurred among various colchicine injection times (F = 10.150, P = 0.004). The most effective stage for colchicine-induced 2n pollen occurred between the zygotene to diakinesis substages. Sixty-eight triploids were confirmed with both flow cytometric analysis and chromosome number-counting among the 3346 offspring. The highest triploid induction rate was 13.2%—significantly lower than the expected triploid production rate of 47.0%—indicating that the colchicine-induced 2n pollen was weak during fertilization. We found positive correlation between the efficiency of triploid production and the frequency of colchicine-induced 2n pollen (r = 0.961, P = 0.001) which suggests that the triploid production rate increased as the occurrence rate of colchicine-induced 2n pollen increased. Analysis using the GLM-univariate indicated that both pollen types (F = 87.50, P < 0.001) and durations of pollen germination (F = 7.61, P = 0.002) did have significant effects on the lengths of pollen-tubes. Haploid pollen-tubes were significantly longer than colchicine-induced 2n pollen-tubes. This suggests that haploid pollen has an advantage during fertilization.
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DOI：
--
发表时间：
2010
期刊：
Journal of Nuclear Agricultural Sciences
影响因子：
--
作者：
Kang Xiangyang
通讯作者：
Kang Xiangyang
二倍体胡杨花粉数量未减少的发生及其细胞学机制
影响因子：
1
作者：
Zhang Pingdong;Kang Xiangyang
通讯作者：
Kang Xiangyang
DOI：
--
发表时间：
2000
期刊：
Journal of Beijing Forestry University
影响因子：
--
作者：
Kang Xiangyang;Zhu Zhi-ti;Z. Zhiyi
通讯作者：
Kang Xiangyang;Zhu Zhi-ti;Z. Zhiyi
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
Kang Xiang-yang
通讯作者：
Kang Xiang-yang
影响因子：
1.9
作者：
Zheng-hai Zhang;X. Kang
通讯作者：
Zheng-hai Zhang;X. Kang