Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds.
Biochemical association of metabolic profile and microbiome in chronic pressure ulcer wounds.
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DOI：
10.1371/journal.pone.0126735
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Copié V
中科院分区：
文献类型：
--
作者：
Ammons MC;Morrissey K;Tripet BP;Van Leuven JT;Han A;Lazarus GS;Zenilman JM;Stewart PS;James GA;Copié V
在免疫系统轻度至严重受损的临床人群中，慢性、不愈合的伤口会极大地加重患有合并症的患者的痛苦。正常的伤口愈合是通过一个明确描述的过程进行的。然而，在慢性伤口中，在炎症消退和上皮细胞再生之间的过渡过程中，这一过程似乎变得失调。定植细菌形式的生物负荷是压疮等慢性伤口延迟出现的主要原因。然而，微生物组如何影响伤口代谢景观尚不清楚。在这里，我们使用系统生物学方法来确定细菌定植的伤口的分类学和代谢组学特征之间的生化关联。从原发性慢性伤口采集压疮活组织检查，并分成顶部和底部部分，然后通过焦磷酸测序分析微生物组，并使用 1H 核磁共振 (NMR) 光谱分析代谢组。细菌分类学显示伤口主要由三个主要门定殖，但在属水平上存在显着差异。虽然分类学特征显示伤口之间存在显着差异，但根据伤口活检的深度，代谢特征具有显着的相似性。分类学和代谢景观之间的生化关联表明，伤口与伤口在代谢物富集集和代谢途径影响方面存在显着相似性，特别是在氨基酸代谢方面。据我们所知，这是首次证明慢性伤口环境中细菌定植与代谢景观之间存在统计学上的稳健相关性。
Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become dysregulated at the transition between resolution of inflammation and re-epithelialization. Bioburden in the form of colonizing bacteria is a major contributor to the delayed headlining in chronic wounds such as pressure ulcers. However how the microbiome influences the wound metabolic landscape is unknown. Here, we have used a Systems Biology approach to determine the biochemical associations between the taxonomic and metabolomic profiles of wounds colonized by bacteria. Pressure ulcer biopsies were harvested from primary chronic wounds and bisected into top and bottom sections prior to analysis of microbiome by pyrosequencing and analysis of metabolome using 1H nuclear magnetic resonance (NMR) spectroscopy. Bacterial taxonomy revealed that wounds were colonized predominantly by three main phyla, but differed significantly at the genus level. While taxonomic profiles demonstrated significant variability between wounds, metabolic profiles shared significant similarity based on the depth of the wound biopsy. Biochemical association between taxonomy and metabolic landscape indicated significant wound-to-wound similarity in metabolite enrichment sets and metabolic pathway impacts, especially with regard to amino acid metabolism. To our knowledge, this is the first demonstration of a statistically robust correlation between bacterial colonization and metabolic landscape within the chronic wound environment.
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影响因子：
3.7
作者：
Bogaert D;Keijser B;Huse S;Rossen J;Veenhoven R;van Gils E;Bruin J;Montijn R;Bonten M;Sanders E
通讯作者：
Sanders E
DOI：
10.1073/pnas.1004204107
发表时间：
2010-08-17
影响因子：
11.1
作者：
Grice, Elizabeth A.;Snitkin, Evan S.;Segre, Julia A.
通讯作者：
Segre, Julia A.
影响因子：
3.1
作者：
Gjodsbol, Kristine;Skindersoe, Mette E.;Krogfelt, Karen A.
通讯作者：
Krogfelt, Karen A.
影响因子：
2.9
作者：
Han, Anne;Zenilman, Jonathan M.;Lazarus, Gerald S.
通讯作者：
Lazarus, Gerald S.
影响因子：
3.2
作者：
Gao, Chao;Hu, Chunhui;Xu, Ping
通讯作者：
Xu, Ping