Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin.
Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin.
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DOI：
10.1038/s41598-020-79378-9
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发表时间：
2021-01-08
影响因子：
4.6
通讯作者：
Elbe-Bürger A
中科院分区：
文献类型：
--
作者：
Seiser S;Janker L;Zila N;Mildner M;Rakita A;Matiasek J;Bileck A;Gerner C;Paulitschke V;Elbe-Bürger A
奥替尼啶二盐酸盐（OCT）是一种广泛使用的抗菌分子，促进皮肤伤口愈合，同时改善手术后的瘢痕质量。然而，OCT促进组织再生的机制尚不完全清楚。在这项研究中，我们使用了一个浅表伤口模型，通过胶带剥离离体人类皮肤。分别使用液相色谱-质谱（LC-MS）和酶联免疫吸附测定（ELISA）分析用含OCT的水凝胶和释放的分泌体处理的受伤皮肤活检组织的蛋白质谱。OCT治疗的皮肤伤口的蛋白质组学分析显示，与对照组相比，组织重塑以及创伤后再上皮化中的关键参与者，如促炎细胞因子（IL-8，IL-6）和基质金属蛋白酶（MMP 1，MMP 2，MMP 3，MMP 9）的水平显著降低。此外，在OCT处理的样品中，几种释放到培养上清液中的MMP的酶活性显著降低。我们的数据提供了关于OCT积极影响伤口愈合的作用模式的见解，并确定了OCT的抗炎和蛋白酶抑制活性。
Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC–MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.
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