Mutation in the Cadherin Gene Is a Key Factor for Pink Bollworm Resistance to Bt Cotton in China.
Mutation in the Cadherin Gene Is a Key Factor for Pink Bollworm Resistance to Bt Cotton in China.
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DOI：
10.3390/toxins14010023
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发表时间：
2022-01-01
期刊：
影响因子：
4.2
通讯作者：
Wan P
中科院分区：
文献类型：
--
作者：
Wang L;Xu D;Huang Y;Zhou H;Liu W;Cong S;Wang J;Li W;Wan P
生产苏云金芽孢杆菌（Bt）毒素的转基因作物被广泛种植用于控制昆虫，但它们的效力可能会随着昆虫产生抗性而下降。了解昆虫抗性的遗传基础对于制定抗性管理的综合战略至关重要。为了解长江流域红铃虫（Pectinophora gossypiella）对Bt棉抗性的遗传基础，首次在F2代进行了与Bt（Cry 1Ac）蛋白抗性相关的等位基因筛选。共筛选出145个有效的单对品系，其中7个品系携带抗性等位基因。这7个品系中的所有田间亲本在钙粘蛋白基因座上携带隐性抗性等位基因，包括3个已知等位基因r1、r13和r15，以及2个新等位基因r19和r20。145个株系的抗性等位基因频率为0.0241（95% CI：0.0106-0.0512）。研究结果表明，长江流域红铃虫对Bt棉的抗性较为罕见，钙粘蛋白基因的隐性突变是该地区红铃虫对Bt棉产生抗性的主要机制，为该地区红铃虫的抗性治理提供了科学依据。
Transgenic crops producing Bacillus thuringiensis (Bt) toxins are widely planted for insect control, but their efficacy may decrease as insects evolve resistance. Understanding the genetic basis of insect resistance is essential for developing an integrated strategy of resistance management. To understand the genetic basis of resistance in pink bollworm (Pectinophora gossypiella) to Bt cotton in the Yangtze River Valley of China, we conducted an F2 screening for alleles associated with resistance to the Bt (Cry1Ac) protein for the first time. A total of 145 valid single-paired lines were screened, among which seven lines were found to carry resistance alleles. All field parents in those seven lines carried recessive resistance alleles at the cadherin locus, including three known alleles, r1, r13 and r15, and two novel alleles, r19 and r20. The overall frequency of resistance alleles in 145 lines was 0.0241 (95% CI: 0.0106–0.0512). These results demonstrated that resistance was rare and that recessive mutation in the cadherin gene was the primary mechanism of pink bollworm resistance to Bt cotton in the Yangtze River Valley of China, which will provide a scientific basis for implementing targeted resistance management statics of pink bollworm in this region.
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多毒素耐药性使金字化BT棉花上的粉红色螺丝虫存活。
影响因子：
4.6
作者：
Fabrick JA;Unnithan GC;Yelich AJ;DeGain B;Masson L;Zhang J;Carrière Y;Tabashnik BE
通讯作者：
Tabashnik BE
DOI：
10.4161/gmcr.20329
发表时间：
2012-07-01
期刊：
GM crops & food
影响因子：
--
作者：
Tabashnik, Bruce E;Morin, Shai;Carriere, Yves
通讯作者：
Carriere, Yves
影响因子：
3.8
作者：
Wang, Ling;Ma, Yuemin;Tabashnik, Bruce E.
通讯作者：
Tabashnik, Bruce E.
DOI：
10.1073/pnas.0831036100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Morin, S;Biggs, RW;Tabashnik, BE
通讯作者：
Tabashnik, BE
影响因子：
2.2
作者：
Tabashnik, Bruce E.;Carriere, Yves
通讯作者：
Carriere, Yves