Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration.
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration.
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DOI：
10.4103/1673-5374.224378
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发表时间：
2018-01
影响因子：
6.1
通讯作者：
da Silva Fernandes MJ
中科院分区：
文献类型：
--
作者：
Fernandes M;Valente SG;Sabongi RG;Gomes Dos Santos JB;Leite VM;Ulrich H;Nery AA;da Silva Fernandes MJ
研究证实骨髓间充质干细胞（MSCs）可用于治疗多种神经系统疾病。然而，骨髓源性间充质干细胞（BMSCs）的分离是一个侵入性和痛苦的过程，产量非常低。因此，有必要寻找其他替代干细胞来源。脂肪源性间充质干细胞（ADSCs）具有与BMSCs相似的表型和基因表达谱。ADSCs的产量大于BMSCs，ADSCs的增殖速度也快于BMSCs。为了比较含有BMSCs或ADSCs的静脉移植物对坐骨神经损伤的影响，本研究将大鼠随机分为4组：假手术（仅暴露坐骨神经）、Matrigel（MG;坐骨神经损伤+静脉移植MG载体）、ADSCs（坐骨神经损伤+静脉内含有ADSCs的MG）和BMSCs（坐骨神经损伤+静脉内含有BMSCs的MG）组。计算坐骨神经功能指数评价坐骨神经损伤后的功能。甲苯胺蓝染色观察远侧神经的形态学特征。用Fluoro-Gold标记的脊髓运动神经元进行定量评估。与假手术组相比，坐骨神经损伤组大鼠坐骨神经功能指数降低，小直径纤维密度明显增加，运动神经元数量明显减少。无论是ADSCs还是BMSCs都没有显著改善坐骨神经损伤大鼠的坐骨神经功能，增加了损伤部位远端坐骨神经的纤维密度、纤维直径、轴突直径、髓鞘厚度和G比（轴突直径/纤维直径比）。ADSC组、BMSCs组和MG组脊髓运动神经元数量差异无统计学意义。这些结果表明，无论是骨髓基质细胞还是脂肪干细胞提供了令人满意的结果，周围神经修复时，使用MG作为导体植入。
Studies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham (only sciatic nerve exposed), Matrigel (MG; sciatic nerve injury + intravenous transplantation of MG vehicle), ADSCs (sciatic nerve injury + intravenous MG containing ADSCs), and BMSCs (sciatic nerve injury + intravenous MG containing BMSCs) groups. Sciatic functional index was calculated to evaluate the function of injured sciatic nerve. Morphologic characteristics of nerves distal to the lesion were observed by toluidine blue staining. Spinal motor neurons labeled with Fluoro-Gold were quantitatively assessed. Compared with sham-operated rats, sciatic functional index was lower, the density of small-diameter fibers was significantly increased, and the number of motor neurons significantly decreased in rats with sciatic nerve injury. Neither ADSCs nor BMSCs significantly improved the sciatic nerve function of rats with sciatic nerve injury, increased fiber density, fiber diameters, axonal diameters, myelin sheath thickness, and G ratios (axonal diameter/fiber diameter ratios) in the sciatic nerve distal to the lesion site. There was no significant difference in the number of spinal motor neurons among ADSCs, BMSCs and MG groups. These results suggest that neither BMSCs nor ADSCs provide satisfactory results for peripheral nerve repair when using MG as the conductor for engraftment.
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DOI：
10.5114/aoms.2011.24127
发表时间：
2011-08
期刊：
Archives of medical science : AMS
影响因子：
--
作者：
Liu GB;Cheng YX;Feng YK;Pang CJ;Li Q;Wang Y;Jia H;Tong XJ
通讯作者：
Tong XJ
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
影响因子：
2.1
作者：
Colonna, M;Anastasi, GP;Tomasello, F
通讯作者：
Tomasello, F
影响因子：
1.5
作者：
LOLLEY, RD;BOSE, WJ;ANDERSON, LD
通讯作者：
ANDERSON, LD
影响因子：
4.1
作者：
Frattini, Flavia;Pereira Lopes, Fatima Rosalina;Blanco Martinez, Ana Maria
通讯作者：
Blanco Martinez, Ana Maria