Adipose-derived stem cells promote peripheral nerve repair.
Adipose-derived stem cells promote peripheral nerve repair.
复制标题
DOI：
10.5114/aoms.2011.24127
复制
发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Tong XJ
中科院分区：
文献类型：
--
作者：
Liu GB;Cheng YX;Feng YK;Pang CJ;Li Q;Wang Y;Jia H;Tong XJ
最近的证据表明，骨髓间充质干细胞的植入改善周围神经再生。在这项研究中，我们的目的是调查是否脂肪来源的干细胞（ADSCs）可以用于周围神经修复。在大鼠模型中，通过使用注射有同种异体ADSC的脱细胞神经，在坐骨神经中的15 mm损伤处评估神经再生。通过足印法测定大鼠的行走行为，并进行电生理分析和组织学检查以评价神经再生的效果。体外扩增后，培养的脂肪干细胞在形态上变得均一，呈双极纺锤形。将ADSC植入大鼠模型导致（i）通过足迹分析测量的改善的行走行为，（ii）腓肠肌和比目鱼肌肌肉质量比的保护增加，（iii）神经传导速度增加，以及（iv）移植物内有髓纤维数量增加。脂肪源性干细胞可促进大鼠周围神经修复。虽然我们实验室正在研究ADSCs促进周围神经再生的详细机制，但我们的研究结果表明，ADSCs移植是周围神经损伤的一种强有力的治疗方法。
Recent evidence suggests that the implantation of bone marrow-derived mesenchymal stem cells improves peripheral nerve regeneration. In this study we aimed to investigate whether adipose-derived stem cells (ADSCs) can be used for peripheral nerve repair. In a rat model, nerve regeneration was evaluated across a 15 mm lesion in the sciatic nerve by using an acellular nerve injected with allogenic ADSCs. The walking behaviour of rats was measured by footprint analysis, and electrophysiological analysis and histological examination were performed to evaluate the efficacy of nerve regeneration. Cultured ADSCs became morphologically homogeneous with a bipolar, spindle-like shape after ex vivo expansion. Implantation of ADSCs into the rat models led to (i) improved walking behaviour as measured by footprint analysis, (ii) increased conservation of muscle-mass ratio of gastrocnemius and soleus muscles, (iii) increased nerve conduction velocity, and (iv) increased number of myelinated fibres within the graft. Adipose-derived stem cells could promote peripheral nerve repair in a rat model. Although the detailed mechanism by which ADSCs promote peripheral nerve regeneration is being investigated in our lab, our results suggest that ADSCs transplantation represents a powerful therapeutic approach for peripheral nerve injury.
登录
查看更多内容
影响因子：
3.3
作者：
Zuk, PA;Zhu, M;Hedrick, MH
通讯作者：
Hedrick, MH
与雪旺细胞共培养是脂肪干细胞神经转分化的有效途径。
DOI：
10.5114/aoms.2010.13885
发表时间：
2010-04-30
期刊：
Archives of medical science : AMS
影响因子：
--
作者：
Liao D;Gong P;Li X;Tan Z;Yuan Q
通讯作者：
Yuan Q
影响因子：
5.3
作者：
DEMEDINACELI, L;FREED, WJ;WYATT, RJ
通讯作者：
WYATT, RJ
影响因子：
5.3
作者：
Chen, Chun-Jung;Ou, Yen-Chuan;Hsu, Shan-Hui
通讯作者：
Hsu, Shan-Hui
影响因子：
2.9
作者：
Wang, Dong;Liu, Xiao-Lin;Yi, Jian-Hua
通讯作者：
Yi, Jian-Hua