Longitudinal observation and decline of neutralizing antibody responses in the three months following SARS-CoV-2 infection in humans.
Longitudinal observation and decline of neutralizing antibody responses in the three months following SARS-CoV-2 infection in humans.
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DOI：
10.1038/s41564-020-00813-8
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发表时间：
2020-12
影响因子：
28.3
通讯作者：
Doores KJ
中科院分区：
文献类型：
--
作者：
Seow J;Graham C;Merrick B;Acors S;Pickering S;Steel KJA;Hemmings O;O'Byrne A;Kouphou N;Galao RP;Betancor G;Wilson HD;Signell AW;Winstone H;Kerridge C;Huettner I;Jimenez-Guardeño JM;Lista MJ;Temperton N;Snell LB;Bisnauthsing K;Moore A;Green A;Martinez L;Stokes B;Honey J;Izquierdo-Barras A;Arbane G;Patel A;Tan MKI;O'Connell L;O'Hara G;MacMahon E;Douthwaite S;Nebbia G;Batra R;Martinez-Nunez R;Shankar-Hari M;Edgeworth JD;Neil SJD;Malim MH;Doores KJ
大多数感染者在COVID-19症状出现后10-15天内可检测到对SARS-CoV-2的抗体（Ab）反应。然而，由于这种病毒最近在人群中出现，目前尚不清楚这些抗体反应将维持多久，或者它们是否会提供防止再次感染的保护。使用从65名RT-qPCR确认的SARS-CoV-2感染者中收集的症状发作（POS）后长达94天的连续血清样本，我们显示>95%的病例发生血清转换，并且当采样超过8天POS时，中和抗体（nAb）应答。我们证明了nAb反应的大小取决于疾病的严重程度，但这并不影响nAb反应的动力学。我们进一步揭示了SARS-CoV-2感染后的nAb反应是典型的急性病毒感染，在初始峰值后观察到nAb滴度下降。虽然一些具有高峰ID 50（> 10，000）的个体在>60天POS时保持nAb滴度> 1，000，但一些具有较低峰值ID 50的个体在随访期内具有接近基线的nAb滴度。在Guy's和St托马斯' Hospitals的血清阳性医护人员队列中也观察到了类似的nAb滴度下降。当考虑到广泛的血清学检测、抗体对SARS-CoV-2再感染的保护以及疫苗诱导保护的持久性时，这项研究具有重要意义。
Antibody (Ab) responses to SARS-CoV-2 can be detected in most infected individuals 10-15 days following the onset of COVID-19 symptoms. However, due to the recent emergence of this virus in the human population it is not yet known how long these Ab responses will be maintained or whether they will provide protection from re-infection. Using sequential serum samples collected up to 94 days post onset of symptoms (POS) from 65 RT-qPCR confirmed SARS-CoV-2-infected individuals, we show seroconversion in >95% of cases and neutralizing antibody (nAb) responses when sampled beyond 8 days POS. We demonstrate that the magnitude of the nAb response is dependent upon the disease severity, but this does not affect the kinetics of the nAb response. We further reveal that the nAb response after SARS-CoV-2 infection is typical of an acute viral infection with declining nAb titres observed following an initial peak. Whilst some individuals with high peak ID50 (>10,000) maintained nAb titres >1,000 at >60 days POS, some with lower peak ID50 had nAb titres approaching baseline within the follow up period. A similar decline in nAb titres was also observed in a cohort of seropositive healthcare workers from Guy’s and St Thomas’ Hospitals. This study has important implications when considering widespread serological testing, Ab protection against re-infection with SARS-CoV-2 and the durability of vaccine-induced protection.
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影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
影响因子：
158.5
作者：
Keech, Cheryl;Albert, Gary;Glenn, Gregory M.
通讯作者：
Glenn, Gregory M.
影响因子：
24.8
作者：
Isho B;Abe KT;Zuo M;Jamal AJ;Rathod B;Wang JH;Li Z;Chao G;Rojas OL;Bang YM;Pu A;Christie-Holmes N;Gervais C;Ceccarelli D;Samavarchi-Tehrani P;Guvenc F;Budylowski P;Li A;Paterson A;Yue FY;Marin LM;Caldwell L;Wrana JL;Colwill K;Sicheri F;Mubareka S;Gray-Owen SD;Drews SJ;Siqueira WL;Barrios-Rodiles M;Ostrowski M;Rini JM;Durocher Y;McGeer AJ;Gommerman JL;Gingras AC
通讯作者：
Gingras AC
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F
影响因子：
56.9
作者：
Chandrashekar, Abishek;Liu, Jinyan;Barouch, Dan H.
通讯作者：
Barouch, Dan H.