Measuring metabolic rate in single flies during sleep and waking states via indirect calorimetry.
Measuring metabolic rate in single flies during sleep and waking states via indirect calorimetry.
复制标题
用间接量热法测量果蝇在睡眠和清醒状态下的代谢率。
DOI：
10.1016/j.jneumeth.2022.109606
复制
发表时间：
2022-07-01
影响因子：
3
通讯作者：
Keene, Alex C.
中科院分区：
文献类型：
--
作者：
Brown, Elizabeth B.;Klok, Jaco;Keene, Alex C.
黑腹果蝇是研究睡眠神经调节的主要遗传模型。睡眠与行为和生理状态的变化有关，这些变化在很大程度上是物种间保守的。对苍蝇睡眠的研究主要集中在睡眠的行为读数上，因为生理测量，包括大脑活动和代谢率的变化，是不太容易获得的。我们以前曾使用停流间接量热法来测量单个苍蝇的全身代谢率，并表明苍蝇和哺乳动物一样，在睡眠期间代谢率降低。在这里，我们描述了一个修改后的版本，该系统允许有效和高度敏感的收购CO2输出从单苍蝇。在这个修改后的系统中，我们发现，睡眠依赖性的代谢率变化在衰老的苍蝇减少，支持睡眠质量随着苍蝇年龄的增长而降低的观点。我们还描述了一种修改，允许同时采集的CO2和O2水平，提供了一个呼吸商，量化如何利用代谢商店。我们发现，在全糖饮食的果蝇中确定的呼吸商提示脂肪生成，其中提供给果蝇的膳食糖被转化为脂肪。总之，通过间接量热法测量代谢率不仅提供了睡眠深度的生理读数，而且还提供了营养利用的代谢调节的洞察力，广泛应用于果蝇的遗传研究。
Drosophila melanogaster is a leading genetic model for studying the neural regulation of sleep. Sleep is associated with changes in behavior and physiological state that are largely conserved across species. The investigation of sleep in flies has predominantly focused on behavioral readouts of sleep because physiological measurements, including changes in brain activity and metabolic rate are less accessible. We have previously used stop-flow indirect calorimetry to measure whole body metabolic rate in single flies and have shown that in flies, like mammals, metabolic rate is reduced during sleep. Here, we describe a modified version of this system that allows for efficient and highly sensitive acquisition of CO2 output from single flies. In this modified system, we show that sleep-dependent changes in metabolic rate are diminished in aging flies, supporting the notion that sleep quality is reduced as flies age. We also describe a modification that allows for simultaneous acquisition of CO2 and O2 levels, providing a respiratory quotient that quantifies how metabolic stores are utilized. We find that the respiratory quotient identified in flies on an all-sugar diet is suggestive of lipogenesis, where the dietary sugar provided to the flies is being converted to fat. Taken together, the measurement of metabolic rate via indirect calorimetry not only provides a physiological readout of sleep depth, but also provides insight the metabolic regulation of nutrient utilization, with broad applications to genetic studies in flies.
登录
查看更多内容
鸟类的环境，迁徙趋势，系统发育和基础代谢率。
影响因子：
3.7
作者：
Jetz, Walter;Freckleton, Robert P.;McKechnie, Andrew E.
通讯作者：
McKechnie, Andrew E.
影响因子：
16.6
作者：
Botero V;Stanhope BA;Brown EB;Grenci EC;Boto T;Park SJ;King LB;Murphy KR;Colodner KJ;Walker JA;Keene AC;Ja WW;Tomchik SM
通讯作者：
Tomchik SM
影响因子：
5.6
作者：
Caron, Aimee M.;Stephenson, Richard
通讯作者：
Stephenson, Richard
DOI：
10.1016/j.cub.2010.05.029
发表时间：
2010-07-13
期刊：
Current biology : CB
影响因子：
--
作者：
Keene AC;Duboué ER;McDonald DM;Dus M;Suh GS;Waddell S;Blau J
通讯作者：
Blau J
影响因子：
1.2
作者：
Bland, Michelle L.
通讯作者：
Bland, Michelle L.