Downregulation of blood serum microRNA 29 family in patients with Parkinson's disease.
Downregulation of blood serum microRNA 29 family in patients with Parkinson's disease.
复制标题
帕金森病患者血清microRNA 29家族的下调
DOI：
10.1038/s41598-017-03887-3
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发表时间：
2017-07-14
影响因子：
4.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Bai X;Tang Y;Yu M;Wu L;Liu F;Ni J;Wang Z;Wang J;Fei J;Wang W;Huang F;Wang J
目前，帕金森病（PD）尚无可靠且易于应用的诊断标志物。本研究旨在比较帕金森病患者与健康对照者血清中微小RNA 29家族（miR - 29s）的表达谱，明确miR - 29s的表达是否与疾病严重程度、病程或左旋多巴治疗相关，以及其表达是否取决于患者的性别和年龄。采用逆转录 - 实时荧光定量聚合酶链反应（PCR）检测80例帕金森病患者和80例健康对照者血清中miR - 29s的水平。PCR产物通过克隆和测序进行验证。此外，还检测了帕金森病患者和对照者血清中miR - 7的表达。与健康对照者相比，帕金森病患者血清miR - 29水平显著下调。女性帕金森病患者血清miR - 29水平明显高于男性患者。血清miR - 29a和miR - 29c的表达随疾病严重程度有降低趋势。此外，研究发现帕金森病患者与对照者血清miR - 7水平无差异。因此，血清miR - 29水平，尤其是miR - 29a和miR - 29c水平的降低，使其作为帕金森病生物标志物的潜力值得进一步研究。
There is currently no reliable and easily applicable diagnostic marker for Parkinson’s disease (PD). The aims of the present study were to compare the expression profiles of the microRNA29 family (miR-29s) in blood serum from patients with PD with healthy controls and to clarify whether the expression of miR-29s is correlated with disease severity, duration or L-dopa therapy and whether expression depends on the gender and age of patients. The levels of blood serum miR-29s in 80 patients with PD and 80 unaffected controls were assessed by reverse transcription-quantitative real-time PCR. The PCR products were confirmed by cloning and sequencing. Additionally, the expression of miR-7 in the blood serum from PD patients and control subjects was assessed. Serum miR-29 levels were significantly downregulated in PD patients compared to healthy controls. The serum miR-29 levels in female PD patients were markedly higher than in male PD patients. The expression of serum miR-29a and miR-29c expression tended to decrease with disease severity. Moreover, we found that serum miR-7 levels did not differ between PD patients and control subjects. Therefore, the reduction of serum miR-29 levels, particularly miR-29a and miR-29c, warrants further investigation of its potential serving as biomarkers for PD.
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影响因子：
10.5
作者：
Kole, Adam J.;Swahari, Vijay;Deshmukh, Mohanish
通讯作者：
Deshmukh, Mohanish
影响因子：
6.3
作者：
Khanna, Savita;Rink, Cameron;Sen, Chandan K.
通讯作者：
Sen, Chandan K.
影响因子：
11
作者：
HUGHES, AJ;DANIEL, SE;LEES, AJ
通讯作者：
LEES, AJ
DOI：
10.1073/pnas.0710263105
发表时间：
2008-04-29
影响因子：
11.1
作者：
Hebert, Sebastien S.;Horre, Katrien;De Strooper, Bart
通讯作者：
De Strooper, Bart
影响因子：
3.7
作者：
Gilad S;Meiri E;Yogev Y;Benjamin S;Lebanony D;Yerushalmi N;Benjamin H;Kushnir M;Cholakh H;Melamed N;Bentwich Z;Hod M;Goren Y;Chajut A
通讯作者：
Chajut A