Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects.
Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects.
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DOI：
10.1016/j.actbio.2011.06.043
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发表时间：
2011-10
影响因子：
9.7
通讯作者：
Kasper, F. K.
中科院分区：
文献类型：
--
作者：
Henslee, A. M.;Spicer, P. P.;Yoon, D. M.;Nair, M. B.;Meretoja, V. V.;Witherel, K. E.;Jansen, J. A.;Mikos, A. G.;Kasper, F. K.
关键词：
本研究以聚富马酸丙二醇酯(PPF)为支架材料，以聚乳酸-乙醇酸共聚物(PLGA)微球为载体，在聚乳酸-乙醇酸共聚物(PLGA)微球表面吸附了重组人骨形态发生蛋白-2(rhBMP-2)，构建了两部分骨组织工程支架。支架植入大鼠股骨临界大小的节段性缺损处，内固定12周。在整个研究过程中，通过X光检查评估骨形成，并通过显微CT和组织学评估安乐死后的骨形成。通过扭转试验进一步评价其机械稳定性。实验植入组包括PPF棒和带有多孔PPF套筒的PPF棒，该棒含有表面吸附了0、2或8μg重组人骨形态发生蛋白-2的PLGA微球。结果表明，支架的存在增加了缺损区的机械稳定性，与空白缺损区相比，使用棒状物增加了股骨的扭转刚度。尽管人工骨形成棒的存在减少了骨形成，但套筒的存在与低剂量和高剂量的重组人骨形态发生蛋白-2联合应用后，扭转刚度分别从0.56±0.24N·mm增加到2.06±0.63N·mm和1.68±0.56N·mm。结果表明，虽然支架可以为再生组织提供结构支持并提高其机械性能，但如果支架存在于缺损处，如果它们干扰细胞过程，可能会阻碍整体骨形成。
In this study, a two part bone tissue engineering scaffold was investigated consisting of a solid poly(propylene fumarate) (PPF) intramedullary rod for mechanical support surrounded by a porous PPF sleeve for osseointegration and delivery of poly(DL-lactic-co-glycolic acid) (PLGA) microspheres with adsorbed recombinant human bone morphogenetic protein-2 (rhBMP-2). Scaffolds were implanted into critical size rat segmental femoral defects with internal fixation for 12 weeks. Bone formation was assessed throughout the study via radiography, and following euthanasia via micro-CT and histology. Mechanical stabilization was evaluated further via torsional testing. Experimental implant groups included the PPF rod alone and the rod with a porous PPF sleeve containing PLGA microspheres with 0, 2, or 8 μg of rhBMP-2 adsorbed onto their surface. Results showed that presence of the scaffold increased mechanical stabilization of the defect, as evidenced by the increased torsional stiffness of the femurs by the presence of a rod compared to the empty defect. Although the presence of a rod decreased bone formation, the presence of a sleeve combined with a low or high dose of rhBMP-2 increased the torsional stiffness to 2.06 ± 0.63 N·mm and 1.68 ± 0.56 N·mm, respectively, from 0.56 ± 0.24 N·mm for the rod alone. The results indicate that, while scaffolds may provide structural support to regenerating tissues and increase their mechanical properties, the presence of scaffolds within defects may hinder overall bone formation if they interfere with cellular processes.
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影响因子：
4.9
作者：
Aronin, Caren E. Petrie;Sadik, Karim W.;Lay, Ann L.;Rion, Dave B.;Tholpady, Sunil S.;Ogle, Roy C.;Botchwey, Edward A.
通讯作者：
Botchwey, Edward A.
影响因子：
4.9
作者：
Hedberg, EL;Kroese-Deutman, HC;Jansen, JA
通讯作者：
Jansen, JA
DOI：
10.1016/s0928-4931(98)00061-7
发表时间：
1998-12-01
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Ito, Y
通讯作者：
Ito, Y
影响因子：
9.7
作者：
Balcik, Cenk;Tokdemir, Turgut;Korkusuz, Feza
通讯作者：
Korkusuz, Feza
影响因子：
1.8
作者：
Chen, D;Zhao, M;Mundy, GR
通讯作者：
Mundy, GR