Comparative effects of scaffold pore size, pore volume, and total void volume on cranial bone healing patterns using microsphere-based scaffolds.
Comparative effects of scaffold pore size, pore volume, and total void volume on cranial bone healing patterns using microsphere-based scaffolds.
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DOI：
10.1002/jbm.a.32015
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发表时间：
2009-06
影响因子：
4.9
通讯作者：
Botchwey, Edward A.
中科院分区：
文献类型：
--
作者：
Aronin, Caren E. Petrie;Sadik, Karim W.;Lay, Ann L.;Rion, Dave B.;Tholpady, Sunil S.;Ogle, Roy C.;Botchwey, Edward A.
由损伤、疾病或出生缺陷引起的骨性颅面缺损仍然是一个相当大的临床挑战。在这项研究中，微球为基础的支架制造方法被用来研究支架孔径，开孔体积，和总空隙体积分数对骨组织浸润和骨再生在一个临界尺寸的大鼠颅骨缺损的各自的影响。为了比较这些参数的愈合效果，制造了三种不同的支架类型：实心100 μm球体、实心500 μm球体和空心500 μm球体。将这些结构植入手术创建的大鼠颅骨缺损中。术后90天，显微计算机断层扫描（CT）分析结果显示，所有支架产生的新骨数量相似，明显大于未处理的对照组。有趣的是，缺损区域内新骨的空间分布因支架组而异。MicroCT和组织学分析表明，空心500 μm组的愈合仅限于硬脑膜侧，而实心500 μm组的愈合沿着硬脑膜侧和缺损中心。实心100 μm组显示沿硬脑膜层、骨膜层和缺损中心沿着愈合。这些结果表明，孔径和闭合空隙体积都可能在支架降解模式和相关的骨愈合中发挥重要作用。
Bony craniofacial deficits resulting from injury, disease, or birth defects remain a considerable clinical challenge. In this study, microsphere-based scaffold fabrication methods were use to study the respective effects of scaffold pore size, open pore volume, and total void volume fraction on osseous tissue infiltration and bone regeneration in a critical size rat cranial defect. To compare the healing effects of these parameters, three different scaffolds types were fabricated: solid 100 μm spheres, solid 500 μm spheres, and hollow 500 μm spheres. These constructs were implanted into surgically created rat calvarial defects. By 90-days post op, results of micro computed tomography (CT) analysis showed that all scaffolds generated similar amounts of new bone which was significantly greater than untreated controls. Interestingly, the spatial distribution of new bone within the defect area varied by scaffold group. MicroCT and histological analysis demonstrated healing restricted to the dural side in the hollow 500 μm group, whereas the solid 500 μm group demonstrated healing along the dural side and within the center of the defect. Solid 100 μm groups demonstrated healing along the dural layer, periosteal layer, and within the center of the defect. These results suggest that pore size and closed void volume may both play important roles in scaffold degradation patterns and associated bone healing.
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