Label-free analysis of the characteristics of a single cell trapped by acoustic tweezers.
Label-free analysis of the characteristics of a single cell trapped by acoustic tweezers.
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DOI：
10.1038/s41598-017-14572-w
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发表时间：
2017-10-26
影响因子：
4.6
通讯作者：
Shung KK
中科院分区：
文献类型：
--
作者：
Kim MG;Park J;Lim HG;Yoon S;Lee C;Chang JH;Shung KK
单细胞分析对于了解细胞的物理和功能特性至关重要。这些特征的基本知识对于阐明各种细胞的独特特征和疾病的致病因素以及确定疾病的最有效治疗方法非常重要。最近，基于紧聚焦超声微束的声镊由于其能够从异质细胞样品中抓取和分离单个细胞并测量其物理特性而引起了相当大的关注。然而，在捕获靶细胞的同时不能执行测量，因为当前的方法使用长超声脉冲来捕获一个细胞，并且使用短脉冲来询问靶细胞。在本文中，我们证明了短的超声脉冲可以用来产生声捕获力相媲美的长脉冲通过调整脉冲重复频率（PRF）。这使我们能够捕获单个细胞并同时测量其物理特性。此外，它示出，在167 kHz的脉冲重复频率的短超声脉冲可以捕获和分离任一个红细胞或一个前列腺癌细胞，并促进其集成的后向散射系数相关的细胞大小和机械性能的同时测量。
Single-cell analysis is essential to understand the physical and functional characteristics of cells. The basic knowledge of these characteristics is important to elucidate the unique features of various cells and causative factors of diseases and determine the most effective treatments for diseases. Recently, acoustic tweezers based on tightly focused ultrasound microbeam have attracted considerable attention owing to their capability to grab and separate a single cell from a heterogeneous cell sample and to measure its physical cell properties. However, the measurement cannot be performed while trapping the target cell, because the current method uses long ultrasound pulses for grabbing one cell and short pulses for interrogating the target cell. In this paper, we demonstrate that short ultrasound pulses can be used for generating acoustic trapping force comparable to that with long pulses by adjusting the pulse repetition frequency (PRF). This enables us to capture a single cell and measure its physical properties simultaneously. Furthermore, it is shown that short ultrasound pulses at a PRF of 167 kHz can trap and separate either one red blood cell or one prostate cancer cell and facilitate the simultaneous measurement of its integrated backscattering coefficient related to the cell size and mechanical properties.
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影响因子：
4.2
作者：
Kim MG;Yoon S;Kim HH;Shung KK
通讯作者：
Shung KK
影响因子：
5.6
作者：
Gross A;Schoendube J;Zimmermann S;Steeb M;Zengerle R;Koltay P
通讯作者：
Koltay P
影响因子：
3.8
作者：
Lee, Jungwoo;Lee, Changyang;Kim, Hyung Ham;Jakob, Anette;Lemor, Robert;Teh, Shia-Yen;Lee, Abraham;Shung, K. Kirk
通讯作者：
Shung, K. Kirk
DOI：
10.1073/pnas.1413325111
发表时间：
2014-09-09
影响因子：
11.1
作者：
Ding, Xiaoyun;Peng, Zhangli;Huang, Tony Jun
通讯作者：
Huang, Tony Jun
影响因子：
2.4
作者：
Baddour, RE;Sherar, MD;Kolios, MC
通讯作者：
Kolios, MC