A Multimodal Desorption Electrospray Ionisation Workflow Enabling Visualisation of Lipids and Biologically Relevant Elements in a Single Tissue Section.
A Multimodal Desorption Electrospray Ionisation Workflow Enabling Visualisation of Lipids and Biologically Relevant Elements in a Single Tissue Section.
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DOI：
10.3390/metabo13020262
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发表时间：
2023-02-11
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
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作者：
元素物种与宿主生物分子（如脂质和代谢物）的共存可能会为代谢途径的失调以及这些途径如何影响疾病发病机制提供新的线索。碱金属一直是广泛研究的主题，与各种神经退行性疾病和感染性疾病有关，并且已知会破坏脂质代谢。解吸电喷雾电离（DESI）是一种广泛使用的分子成像方法，但以前的工作表明，DESI离域离子，如钾（K）和氯（Cl），排除了随后的元素分析的同一部分组织。通常用于DESI电喷雾的溶剂是甲醇和水的组合。在这里，我们表明，一种新的溶剂系统，（50：50（%v/v）甲醇：乙醇）不离域元素物种，从而使元素映射后，DESI相同的组织切片上进行。将MeOH：EtOH电喷雾溶剂与广泛使用的MeOH：H2O电喷雾溶剂进行基准测试显示，MeOH：EtOH溶剂对所选脂质产生增加的信噪比。所开发的多模态成像工作流程应用于包含结核肉芽肿的肺组织切片，展示了其对显示定义的结构信息的元素丰富的样本的适用性。
The colocation of elemental species with host biomolecules such as lipids and metabolites may shed new light on the dysregulation of metabolic pathways and how these affect disease pathogeneses. Alkali metals have been the subject of extensive research, are implicated in various neurodegenerative and infectious diseases and are known to disrupt lipid metabolism. Desorption electrospray ionisation (DESI) is a widely used approach for molecular imaging, but previous work has shown that DESI delocalises ions such as potassium (K) and chlorine (Cl), precluding the subsequent elemental analysis of the same section of tissue. The solvent typically used for the DESI electrospray is a combination of methanol and water. Here we show that a novel solvent system, (50:50 (%v/v) MeOH:EtOH) does not delocalise elemental species and thus enables elemental mapping to be performed on the same tissue section post-DESI. Benchmarking the MeOH:EtOH electrospray solvent against the widely used MeOH:H2O electrospray solvent revealed that the MeOH:EtOH solvent yielded increased signal-to-noise ratios for selected lipids. The developed multimodal imaging workflow was applied to a lung tissue section containing a tuberculosis granuloma, showcasing its applicability to elementally rich samples displaying defined structural information.
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影响因子：
1.3
作者：
Kump, P.;Vogel-Mikus, K.
通讯作者：
Vogel-Mikus, K.
DOI：
10.1021/jasms.2c00226
发表时间：
2022-12-07
影响因子：
3.2
作者：
Costa, Catia;de Jesus, Janella;Nikula, Chelsea;Murta, Teresa;Grime, Geoffrey W.;Palitsin, Vladimir;Webb, Roger;Goodwin, Richard J. A.;Bunch, Josephine;Bailey, Melanie Jane
通讯作者：
Bailey, Melanie Jane
影响因子：
4.3
作者：
Bowman, Andrew P.;Bogie, Jeroen F. J.;Ellis, Shane R.
通讯作者：
Ellis, Shane R.
影响因子：
15
作者：
Grime, Geoffrey W.;Zeldin, Oliver B.;Garman, Elspeth F.
通讯作者：
Garman, Elspeth F.
DOI：
10.1007/978-1-4939-1258-2_20
发表时间：
2014-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Eberlin, Livia S
通讯作者：
Eberlin, Livia S