The Kaposi sarcoma-associated herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients.
The Kaposi sarcoma-associated herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients.
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DOI：
10.1084/jem.184.1.283
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发表时间：
1996-07-01
影响因子：
15.3
通讯作者：
ThorleyLawson, DA
中科院分区：
文献类型：
--
作者：
Decker, LL;Shankar, P;Khan, G;Freeman, RB;Dezube, BJ;Lieberman, J;ThorleyLawson, DA
短DNA序列已被鉴定，最初与卡波西肉瘤（KS）活检相关，与致癌性嗜淋巴细胞疱疹病毒高度同源。最近，已经在源自体腔淋巴瘤的细胞系中鉴定了携带这些序列的病毒，卡波西肉瘤相关疱疹病毒（KSHV）或人疱疹病毒-8（HHV-8）。在这份报告中，我们表明，相同的序列存在于KS活检的DNA分子的形式和大小的特点，潜伏性疱疹病毒大，共价封闭，环状附加体。基因组迁移的明显大小大于疱疹病毒Epstein-Barr病毒（172 kb）。这种形式的病毒基因组在KS患者的四份活检中的四份和五份外周血样本中的三份中发现。线性形式的病毒基因组，病毒复制的特点，没有检测到活组织检查，但存在于外周血中的五分之三的患者。KSHV/HHV-8的序列也在五名同种异体移植患者中的四名和五名健康供体中的三名的血液中检测到，这表明该病毒在整个人群中广泛传播。
Short DNA sequences have been identified, originally in association with Kaposi's sarcoma (KS) biopsies, that are highly homologous to oncogenic, lymphotropic herpesviruses. Recently a virus, Kaposi sarcoma associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8), bearing these sequences has been identified in a cell line derived from a body cavity-based lymphoma. In this report, we show that the same sequences are present in KS biopsies as DNA molecules of a form and size characteristic of latent herpesviruses-large, covalently closed, circular episomes. The genomes migrate with an apparent size larger than the herpesvirus Epstein-Barr virus (172 kb). This form of the viral genome was found in four of four biopsies and three of five peripheral blood samples from KS patients. Linear forms of the viral genome, characteristic of viral replication, were not detected in the biopsies, but were present in the peripheral blood of three out of five patients. The sequences for KSHV/HHV-8 were also detected in the blood of four of five allograft patients and three of five healthy donors without KS suggesting that the virus is widespread throughout the human population.
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DOI：
10.1038/newbio233103a0
发表时间：
1971-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
NONOYAMA, M;PAGANO, JS
通讯作者：
PAGANO, JS
影响因子：
20.3
作者：
CESARMAN, E;MOORE, PS;CHANG, Y
通讯作者：
CHANG, Y
影响因子：
64.5
作者：
MIYASHITA, EM;YANG, B;THORLEYLAWSON, DA
通讯作者：
THORLEYLAWSON, DA
影响因子：
158.5
作者：
MOORE, PS;CHANG, Y
通讯作者：
CHANG, Y
影响因子：
56.9
作者：
AMBROZIAK, JA;BLACKBOURN, DJ;LEVY, JA
通讯作者：
LEVY, JA