Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation.
Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation.
复制标题
PtoMYB74 在杨树和拟南芥中的异位表达促进次生细胞壁形成
DOI：
10.3389/fpls.2018.01262
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Luo K
中科院分区：
文献类型：
--
作者：
Li C;Ma X;Yu H;Fu Y;Luo K
在维管植物中，R2 R3-MYB转录因子是次生细胞壁形成的重要调节因子。虽然在白杨基因组中鉴定了192个注释的R2 R3 MYB基因，但只有少数成员被表征为参与次生细胞壁生物合成的调控。在本文中，我们鉴定了一个R2 R3-MYB转录因子PtoMYB 74，预计它是拟南芥AtMYB 61的直系同源物，AtMYB 61是一种转录激活因子，调节次生细胞壁形成、木质素生物合成、气孔孔径和种皮粘液。PtoMYB 74主要在茎中表达，尤其是在木质部组织和器官中。PtoMYB 74作为转录激活因子定位于细胞核。PtoMYB 74基因的过表达增加了转基因白杨导管次生细胞壁的厚度，改变了次生细胞壁的组成。与野生型相比，过量表达PtoMYB 74的转基因白杨中木质素和纤维素生物合成基因的表达水平升高，而木聚糖生物合成基因没有变化。转录激活实验表明PtoMYB 74能够激活木质素和纤维素生物合成途径中结构基因的启动子。综上所述，我们的数据表明，PtoMYB 74正调控次生细胞壁的生物合成在白杨。
In vascular plants, R2R3-MYB transcription factors are important regulators of secondary cell wall formation. Although 192 annotated R2R3 MYB genes were identified in the poplar genome, only a few members were characterized to participate in the regulation of the secondary cell wall biosynthesis. In this paper, we identify an R2R3-MYB transcription factor, PtoMYB74, which is predicted to be an ortholog of Arabidopsis AtMYB61, a transcription activator that regulates the secondary cell wall formation, lignin biosynthesis, stomatal aperture, and the mucilage of seed coat. PtoMYB74 is mainly expressed in the stems, especially in the xylem tissues and organs. PtoMYB74 as a transcriptional activator is localized to the nucleus. The overexpression of PtoMYB74 increased the secondary cell wall thickness of vessels in transgenic poplar and changed the secondary cell wall compositions. The expression levels of lignin and cellulose biosynthetic genes were elevated in the transgenic poplar overexpressing PtoMYB74 compared to the wild type, while there was no change in the xylan biosynthetic genes. Transcriptional activation assays demonstrated that PtoMYB74 could activate the promoters of structural genes in the lignin and cellulose biosynthetic pathways. Taken together, our data show that PtoMYB74 positively regulates the secondary cell wall biosynthesis in poplar.
登录
查看更多内容
影响因子：
6.9
作者：
Bomal, Claude;Bedon, Frank;Caron, Sebastien;Mansfield, Shawn D.;Levasseur, Caroline;Cooke, Janice E. K.;Blais, Sylvie;Tremblay, Laurence;Morency, Marie-Josee;Pavy, Nathalie;Grima-Pettenati, Jacqueline;Seguin, Armand;MacKay, John
通讯作者：
MacKay, John
PtoMYB92 是毛白杨次生细胞壁形成过程中木质素生物合成途径的转录激活剂。
影响因子：
4.9
作者：
Li, Chaofeng;Wang, Xianqiang;Luo, Keming
通讯作者：
Luo, Keming
影响因子：
10.5
作者：
Kubo, M;Udagawa, M;Demura, T
通讯作者：
Demura, T
影响因子：
9.2
作者：
Liang, YK;Dubos, C;Campbell, MM
通讯作者：
Campbell, MM
转基因毛白杨 Carr 对真菌病原体的抵抗力增强。
影响因子：
4
作者：
Jia, Zhichun;Gou, Jiqing;Luo, Keming
通讯作者：
Luo, Keming