Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype.
Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype.
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DOI：
10.1186/s43897-022-00025-0
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发表时间：
2022-02-08
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灌木状根-2（brt-2）番茄突变体具有扭曲的根，并且植物发育较慢。在这里，我们使用全基因组重测序和遗传图谱显示，brt-2是由丝氨酸半胱氨酸（S75 C）取代的DNA结合域（DBD）的热休克因子类B（HsfB）由SolycHsfB 4 a编码。该基因与拟南芥SCHIZORIZA基因（也称为AtHsfB 4）是直向同源的。brt-2的表型与AtHsfB 4的功能被改变的拟南芥品系非常相似：侧根帽和根分生组织的增殖，以及侧根帽细胞容易分离的趋势。brt-2的S75 C突变是不寻常的，因为在真核热休克因子的高度保守的DBD中所有其他报道的氨基酸替换都是显性负突变，但brt-2是隐性的。我们进一步表明，通过相互嫁接，BRT-2发挥其作用主要是通过根基因型，甚至通过BRT-2在根和芽分生组织中以相似的水平表达。由于AtHsfB 4是由根结线虫（RKN）诱导的，并且该基因的功能缺失突变体对RKN具有抗性，因此BRT-2可能是番茄砧木抗RKN的一个重要性状的靶基因。 基因和登录号SolycHsfB 4a-Solyc 04 g 078770。在线版本包含补充材料，可通过10.1186/s43897-022-00025-0获得。
The bushy root-2 (brt-2) tomato mutant has twisting roots, and slower plant development. Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine (S75C) substitution in the DNA binding domain (DBD) of a heat shock factor class B (HsfB) encoded by SolycHsfB4a. This gene is orthologous to the Arabidopsis SCHIZORIZA gene, also known as AtHsfB4. The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered: a proliferation of lateral root cap and root meristematic tissues, and a tendency for lateral root cap cells to easily separate. The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations, but brt-2 is recessive. We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems. Since AtHsfB4 is induced by root knot nematodes (RKN), and loss-of-function mutants of this gene are resistant to RKNs, BRT-2 could be a target gene for RKN resistance, an important trait in tomato rootstock breeding. Gene & accession numbers SolycHsfB4a - Solyc04g078770. The online version contains supplementary material available at 10.1186/s43897-022-00025-0.
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影响因子：
56.9
作者：
HARRISON, CJ;BOHM, AA;NELSON, HCM
通讯作者：
NELSON, HCM
影响因子：
3.1
作者：
CLAYBERG, CD;BUTLER, L;ROBINSON, RW
通讯作者：
ROBINSON, RW
影响因子：
2.8
作者：
Galvez, Amparo;del Amor, Francisco M.;Lopez-Marin, Josefa
通讯作者：
Lopez-Marin, Josefa
影响因子：
2.3
作者：
Hammer, Graeme L.;Dong, Zhanshan;Cooper, Mark
通讯作者：
Cooper, Mark
影响因子：
2.6
作者：
Begum, Tahmina;Reuter, Rolf;Schoeffl, Friedrich
通讯作者：
Schoeffl, Friedrich