Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.).
Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.).
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辣椒（Capsicum annuum L.）细胞质雄性不育系及其近等基因恢复系的转录组测序和从头分析
DOI：
10.1371/journal.pone.0065209
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shen HL
中科院分区：
文献类型：
--
作者：
Liu C;Ma N;Wang PY;Fu N;Shen HL
细胞质雄性不育在辣椒F1代杂交制种中的应用日益广泛。然而，细胞质雄性不育和育性恢复的分子机制仍然知之甚少，由于有限的转录组学和基因组数据。因此，本研究利用新一代测序技术（NGS）分析了121 A和121 C的转录组差异，旨在寻找与雄性不育相关的关键基因和途径。我们产生了大约5300万个测序读段并从头组装，产生了85，144个平均长度为643 bp的高质量unigenes。在这些unigenes中，27，191个被鉴定为公共蛋白质数据库中注释序列的推定同源物，4，326个和7，061个unigenes分别被发现在品系121 A和121 C中高度丰富。许多差异表达的单基因代表了一组与花粉形成或败育相关的潜在候选基因。本研究对辣椒不育系及其恢复系的花药转录组进行了分析。这一结果揭示了核-线粒体相互作用紊乱的发生和恢复，并为进一步的研究提供了线索。
The use of cytoplasmic male sterility (CMS) in F1 hybrid seed production of chili pepper is increasingly popular. However, the molecular mechanisms of cytoplasmic male sterility and fertility restoration remain poorly understood due to limited transcriptomic and genomic data. Therefore, we analyzed the difference between a CMS line 121A and its near-isogenic restorer line 121C in transcriptome level using next generation sequencing technology (NGS), aiming to find out critical genes and pathways associated with the male sterility. We generated approximately 53 million sequencing reads and assembled de novo, yielding 85,144 high quality unigenes with an average length of 643 bp. Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively. Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen. Our study profiled anther transcriptomes of a chili pepper CMS line and its restorer line. The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations.
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影响因子：
--
作者：
Guo Shuang;Ma Ning;Shen Huo-lin
通讯作者：
Shen Huo-lin
影响因子：
5.4
作者：
Akagi, H;Nakamura, A;Fujimura, T
通讯作者：
Fujimura, T
影响因子：
4.4
作者：
Blanca J;Cañizares J;Roig C;Ziarsolo P;Nuez F;Picó B
通讯作者：
Picó B
影响因子：
7.2
作者：
Brown, GG;Formanová, N;Landry, BS
通讯作者：
Landry, BS
影响因子：
11.6
作者：
BEGU, D;GRAVES, PV;ARAYA, A
通讯作者：
ARAYA, A