Short- and Long-Term Study of the Impact of Focal Blue Light-Emitting Diode-Induced Phototoxicity in Adult Albino Rats.
Short- and Long-Term Study of the Impact of Focal Blue Light-Emitting Diode-Induced Phototoxicity in Adult Albino Rats.
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对成年白化病大鼠局灶性蓝光发光二极管诱导的光毒性的影响的短期和长期研究。
DOI：
10.3390/ijms22189742
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发表时间：
2021-09-09
影响因子：
5.6
通讯作者：
Vidal-Sanz M
中科院分区：
文献类型：
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作者：
Miralles de Imperial-Ollero JA;Gallego-Ortega A;Norte-Muñoz M;Di Pierdomenico J;Bernal-Garro JM;Valiente-Soriano FJ;Vidal-Sanz M
背景：在成年大鼠中，我们研究了焦点蓝色发光二极管（LED）诱导的光毒性（LIP）对视网膜厚度和Iba-1+激活的短期和长期影响。方法：将已适应黑暗的SD大鼠左眼置于蓝色LED （20 s, 200 lux）下。使用SD-OCT对LIP术后1 ~ 30天进行视网膜厚度、眼底图像和视网膜光学切片的体内纵向监测。在体外，我们分析了S-cone和Iba-1+细胞在病变中心的预定固定大小的圆形区域（PCA）内的种群。结果：LIP导致一个圆形局灶性病变，通过眼底检查在体内很容易识别，在PCAs中显示视网膜外层逐渐变薄，s锥细胞数量减少到19%，持续30天。与s锥丢失平行，激活的Iba-1+细胞描绘了病变区域，并在LIP后第3天获得了峰值表达的变形虫形态。Iba-1+细胞在7 d时呈现较松弛的分支形态，到14-30 d时形态完全分支。结论：LIP引起外视网膜进行性缩小，伴有S锥细胞的丧失和损伤区域小胶质细胞的平行动态激活。
Background: In adult rats we study the short- and long-term effects of focal blue light-emitting diode (LED)-induced phototoxicity (LIP) on retinal thickness and Iba-1+ activation. Methods: The left eyes of previously dark-adapted Sprague Dawley (SD) rats were photoexposed to a blue LED (20 s, 200 lux). In vivo longitudinal monitoring of retinal thickness, fundus images, and optical retinal sections was performed from 1 to 30 days (d) after LIP with SD-OCT. Ex vivo, we analysed the population of S-cone and Iba-1+ cells within a predetermined fixed-size circular area (PCA) centred on the lesion. Results: LIP resulted in a circular focal lesion readily identifiable in vivo by fundus examination, which showed within the PCAs a progressive thinning of the outer retinal layer, and a diminution of the S-cone population to 19% by 30 d. In parallel to S-cone loss, activated Iba-1+ cells delineated the lesioned area and acquired an ameboid morphology with peak expression at 3 d after LIP. Iba-1+ cells adopted a more relaxed-branched morphology at 7 d and by 14–30 d their morphology was fully branched. Conclusion: LIP caused a progressive reduction of the outer retina with loss of S cones and a parallel dynamic activation of microglial cells in the lesioned area.
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影响因子：
--
作者：
Klein, Ronald;Cruickshanks, Karen J.;Nash, Scott D.;Krantz, Elizabeth M.;Javier Nieto, F.;Huang, Guan H.;Pankow, James S.;Klein, Barbara E. K.
通讯作者：
Klein, Barbara E. K.
影响因子：
3.6
作者：
García-Layana A;Cabrera-López F;García-Arumí J;Arias-Barquet L;Ruiz-Moreno JM
通讯作者：
Ruiz-Moreno JM
影响因子：
2.9
作者：
Valiente-Soriano FJ;García-Ayuso D;Ortín-Martínez A;Jiménez-López M;Galindo-Romero C;Villegas-Pérez MP;Agudo-Barriuso M;Vugler AA;Vidal-Sanz M
通讯作者：
Vidal-Sanz M
影响因子：
3.7
作者：
Ortín-Martínez A;Valiente-Soriano FJ;García-Ayuso D;Alarcón-Martínez L;Jiménez-López M;Bernal-Garro JM;Nieto-López L;Nadal-Nicolás FM;Villegas-Pérez MP;Wheeler LA;Vidal-Sanz M
通讯作者：
Vidal-Sanz M
影响因子：
3.3
作者：
Krigel, A.;Berdugo, M.;Behar-Cohen, F.
通讯作者：
Behar-Cohen, F.