Quantum dots-labeled polymeric scaffolds for in vivo tracking of degradation and tissue formation.
Quantum dots-labeled polymeric scaffolds for in vivo tracking of degradation and tissue formation.
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DOI：
10.1016/j.bioactmat.2022.03.003
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发表时间：
2022-10
影响因子：
18.9
通讯作者：
Lee CH
中科院分区：
文献类型：
--
作者：
Sim KH;Mir M;Jelke S;Tarafder S;Kim J;Lee CH
生物材料的体外和体内降解速率之间不可避免的差距已经成为支架降解与新组织形成平衡的最佳设计中的一个挑战性因素。为了能够对体内支架降解进行无创/微创跟踪，已经将化学修饰应用于用荧光染料标记聚合物。然而，由于合成过程复杂，以前的方法可能具有有限的可扩展性。在这里，我们介绍了一种简单而有效的方法，通过与近红外（NIR）量子点（QD），具有上级的光学性能的半导体纳米晶体混合的聚合物支架的荧光标记。QD标记的3D打印PCL支架在使用定制的光纤成像模式定量测量降解方面表现出有希望的效率和可靠性。此外，QDs-PCL支架既不显示细胞毒性，也不显示相邻细胞的二次标记。QDs-PCL支架还支持从间充质干/祖细胞（MSC）工程化纤维、软骨和成骨组织。此外，QDs-PCL能够通过多通道成像区分新形成的组织和支架的剩余质量。因此，我们的研究结果表明，PCL支架和微创成像模式的简单而有效的量子点标记显示出显着的潜力，使支架降解以及新的组织形成的体内跟踪。我们设计了一种简单的荧光标记PCL与NIR量子点具有独特的光学性质和无细胞毒性。光纤成像系统实现了QDs-PCL支架的深层组织成像，用于定量降解测量。NIR信号强度显示与支架的剩余质量的可靠相关性。量子点标记减少PCL自体荧光，使新的组织形成和支架降解的定量。我们的发现对基于生物材料的组织再生具有重要意义。
The inevitable gap between in vitro and in vivo degradation rate of biomaterials has been a challenging factor in the optimal designing of scaffold's degradation to be balanced with new tissue formation. To enable non-/minimum-invasive tracking of in vivo scaffold degradation, chemical modifications have been applied to label polymers with fluorescent dyes. However, the previous approaches may have limited expandability due to complicated synthesis processes. Here, we introduce a simple and efficient method to fluorescence labeling of polymeric scaffolds via blending with near-infrared (NIR) quantum dots (QDs), semiconductor nanocrystals with superior optical properties. QDs-labeled, 3D-printed PCL scaffolds showed promising efficiency and reliability in quantitative measurement of degradation using a custom-built fiber-optic imaging modality. Furthermore, QDs-PCL scaffolds showed neither cytotoxicity nor secondary labeling of adjacent cells. QDs-PCL scaffolds also supported the engineering of fibrous, cartilaginous, and osteogenic tissues from mesenchymal stem/progenitor cells (MSCs). In addition, QDs-PCL enabled a distinction between newly forming tissue and the remaining mass of scaffolds through multi-channel imaging. Thus, our findings suggest a simple and efficient QDs-labeling of PCL scaffolds and minimally invasive imaging modality that shows significant potential to enable in vivo tracking of scaffold degradation as well as new tissue formation. We devised a simple fluorescence labeling of PCL with NIR QDs with unique optical properties and no cytotoxicity. Fiber-optic imaging system enabled deep-tissue imaging of QDs-PCL scaffolds for quantitative degradation measurement. NIR signal intensity showed a solid correlation with the remaining mass of scaffolds. QDs labeling diminished PCL autofluorescence enabling quantification of new tissue formation and scaffold degradation. Our findings have significant implications in biomaterial-based tissue regeneration.
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影响因子：
4.7
作者：
Jing, Linzhi;Sun, Mingtai;Huang, Dejian
通讯作者：
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影响因子：
3
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DOI：
10.1073/pnas.1421632111
发表时间：
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影响因子：
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