Plasma cytokines in women with chronic fatigue syndrome.
Plasma cytokines in women with chronic fatigue syndrome.
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DOI：
10.1186/1479-5876-7-96
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发表时间：
2009-11-12
影响因子：
7.4
通讯作者：
Klimas NG
中科院分区：
文献类型：
--
作者：
Fletcher MA;Zeng XR;Barnes Z;Levis S;Klimas NG
我们实验室和其他实验室的慢性疲劳综合征（CFS）研究描述了细胞因子异常。其他研究报告CFS和对照组之间没有差异。然而，方法学差异很大，很少有研究测量超过4或5种细胞因子。多重技术可以同时测定大量细胞因子，具有高灵敏度，并且每份样本只需30 ul血浆。没有广泛接受的实验室检测或标记物可用于CFS的诊断或预后。本研究筛选血浆因子以鉴定与CFS相关的循环生物标志物。测量女性CFS病例和女性健康对照的血浆中的细胞因子。多重技术提供了16种血浆因子的谱，包括促炎细胞因子：肿瘤坏死因子α（TNFα）、巨噬细胞毒素α（LTα）、白细胞介素（IL）-IL-1 α、IL-1β、IL-6，TH 1细胞因子：干扰素γ（IFNγ）、IL-12 p70、IL-2、IL-15，TH 2细胞因子：IL-4、IL-5，TH 17细胞因子：IL-17、IL-23，TH 18细胞因子：IL-18、IL-19、IL-19。抗炎细胞因子IL-10、IL-13;炎症介质和中性粒细胞吸引趋化因子IL-8（CXCL 8）。通过受试者工作特征（ROC）曲线分析评估了每种细胞因子的生物标志物潜力。与对照组相比，CFS中以下细胞因子升高：LTα、IL-1α、IL-1β、IL-4、IL-5、IL-6和IL-12。CFS中以下细胞因子降低：IL-8、IL-13和IL-15。以下细胞因子无差异：TNFα、IFNγ、IL-2、IL-10、IL-23和IL-17。应用（ROC）曲线分析，IL-5（0. IL-4（0.77）、IL-12（0.76）、LTα（0.84）显示了良好的生物标志物潜力。IL-6（0.73）、IL-15（0.73）、IL-8（0.69）、IL-13（0.68）、IL-1α（0.62）、IL-1β（0.62）的AUC显示出作为生物标志物的良好潜力。细胞因子异常在CFS中很常见。在这项研究中，检查的16种细胞因子中有10种显示出作为生物标志物的良好前景。然而，观察到的细胞因子变化可能更多地指示免疫激活和炎症，而不是CFS的特异性。因此，他们是hereditary战略的目标。较新的技术允许以具有成本效益的方式评估大组细胞因子。
Chronic Fatigue Syndrome (CFS) studies from our laboratory and others have described cytokine abnormalities. Other studies reported no difference between CFS and controls. However, methodologies varied widely and few studies measured more than 4 or 5 cytokines. Multiplex technology permits the determination of cytokines for a large panel of cytokines simultaneously with high sensitivity and with only 30 ul of plasma per sample. No widely accepted laboratory test or marker is available for the diagnosis or prognosis of CFS. This study screened plasma factors to identify circulating biomarkers associated with CFS. Cytokines were measured in plasma from female CFS cases and female healthy controls. Multiplex technology provided profiles of 16 plasma factors including the pro -inflammatory cytokines: tumor necrosis factor α (TNFα), lymphotoxin α (LTα), interleukin (IL) - IL-Iα, IL-1β, IL-6; TH1 cytokines: interferon γ (IFNγ), IL-12p70, IL-2, IL-15; TH2: IL-4, IL-5; TH17 cytokines, IL-17 and IL-23; anti-inflammatory cytokines IL-10, IL-13; the inflammatory mediator and neutrophil attracting chemokine IL-8 (CXCL8). Analysis by receiver operating characteristic (ROC) curve assessed the biomarker potential of each cytokine. The following cytokines were elevated in CFS compared to controls: LTα, IL-1α, IL-1β, IL-4, IL-5, IL-6 and IL-12. The following cytokines were decreased in CFS: IL-8, IL-13 and IL-15. The following cytokines were not different: TNFα, IFNγ, IL-2, IL-10, IL-23 and IL-17. Applying (ROC) curve analyses, areas under the curves (AUC) for IL-5 (0. 84), LTα (0.77), IL-4 (0.77), IL-12 (0.76) indicated good biomarker potential. The AUC of IL-6 (0.73), IL-15 (0.73), IL-8 (0.69), IL-13 (0.68) IL-1α (0.62), IL-1β (0.62) showed fair potential as biomarkers. Cytokine abnormalities are common in CFS. In this study, 10 of 16 cytokines examined showed good to fair promise as biomarkers. However, the cytokine changes observed are likely to more indicative of immune activation and inflammation, rather than specific for CFS. As such, they are targets for herapeutic strategies. Newer techniques allow evaluation of large panels of cytokines in a cost effective fashion.
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影响因子：
56.9
作者：
Lombardi, Vincent C.;Ruscetti, Francis W.;Mikovits, Judy A.
通讯作者：
Mikovits, Judy A.
影响因子：
--
作者：
BOMBARDIER, CH;BUCHWALD, D
通讯作者：
BUCHWALD, D
影响因子：
39.2
作者：
STRAUS, SE;TOSATO, G;BLAESE, RM
通讯作者：
BLAESE, RM
影响因子：
8.7
作者：
Boniface K;Blom B;Liu YJ;de Waal Malefyt R
通讯作者：
de Waal Malefyt R
影响因子：
--
作者：
Jason, LA;Richman, JA;Plioplys, S
通讯作者：
Plioplys, S