A galectin from the kuruma shrimp (Marsupenaeus japonicus) functions as an opsonin and promotes bacterial clearance from hemolymph.
A galectin from the kuruma shrimp (Marsupenaeus japonicus) functions as an opsonin and promotes bacterial clearance from hemolymph.
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来自车虾（Marsupenaeus japonicus）的半乳糖凝集素具有调理素的功能，可促进血淋巴中的细菌清除
DOI：
10.1371/journal.pone.0091794
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang JX
中科院分区：
文献类型：
--
作者：
Shi XZ;Wang L;Xu S;Zhang XW;Zhao XF;Vasta GR;Wang JX
半乳糖凝集素是一个凝集素家族，其特征在于碳水化合物识别结构域中的保守序列基序，其优先结合半乳糖基部分。然而，除了来自东方牡蛎的半乳糖凝集素（CvGal 1）外，很少有关于半乳糖凝集素在无脊椎动物中的生物学作用的研究报道。此外，半乳糖凝集素仅在少数甲壳类物种中被描述，迄今为止还没有功能研究的报道。在这项研究中，我们确定和功能特点的半乳糖凝集素从库鲁马虾囊对虾，我们指定MjGal。鳗弧菌攻击后，MjGal主要在血细胞和肝胰腺中表达上调，并分别通过识别脂磷壁酸（LTA）和脂多糖（LPS）与革兰氏阳性菌和革兰氏阴性菌结合。通过结合到虾血细胞表面，MjGal作为微生物病原体的调理素，促进它们的吞噬作用。此外，如RNA干扰所示，MjGal参与清除循环中的细菌，从而有助于虾针对感染性挑战的免疫防御。阐明虾免疫的功能和机制方面，将使新的策略，目前影响世界各地的虾养殖业的传染病的干预发展。
Galectins are a lectin family characterized by a conserved sequence motif in the carbohydrate recognition domain, which preferential binds to galactosyl moieties. However, few studies about the biological roles of galectins in invertebrates have been reported except for the galectin (CvGal1) from the eastern oyster Crassostrea virginica. Furthermore, galectins have been described in only a few crustacean species, and no functional studies have been reported so far. In this study, we identified and functionally characterized a galectin from the kuruma shrimp Marsupenaeus japonicus, which we designated MjGal. Upon Vibrio anguillarum challenge, expression of MjGal was up-regulated mostly in hemocytes and hepatopancreas, and the protein bound to both Gram-positive and Gram-negative bacteria through the recognition of lipoteichoic acid (LTA) or lipopolysaccharide (LPS), respectively. By also binding to the shrimp hemocyte surface, MjGal functions as an opsonin for microbial pathogens, promoting their phagocytosis. Further, as shown by RNA interference, MjGal participates in clearance of bacteria from circulation, and thereby contributes to the shrimp’s immune defense against infectious challenge. Elucidation of functional and mechanistic aspects of shrimp immunity will enable the development of novel strategies for intervention in infectious diseases currently affecting the shrimp farming industry worldwide.
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影响因子：
20.3
作者：
Stowell, Sean R.;Karmakar, Sougata;Cummings, Richard D.
通讯作者：
Cummings, Richard D.
影响因子：
3.3
作者：
Stowell, Sean R.;Karmakar, Sougata;Cummings, Richard D.
通讯作者：
Cummings, Richard D.
甲状腺素在感染中的作用。
DOI：
10.1038/nrmicro2146
发表时间：
2009-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.9
作者：
Kim, Jin Young;Kim, Young Mee;Cho, Moonjae
通讯作者：
Cho, Moonjae
DOI：
10.1083/jcb.106.5.1795
发表时间：
1988-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Johansson MW;Söderhäll K
通讯作者：
Söderhäll K