Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae.
Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae.
复制标题
DOI：
10.1016/j.ntt.2012.04.010
复制
发表时间：
2012-07
影响因子：
2.9
通讯作者：
Creton, Robbert
中科院分区：
文献类型：
--
作者：
Richendrfer, Holly;Pelkowski, Sean D.;Colwill, Ruth M.;Creton, Robbert
神经行为障碍如焦虑症、自闭症和注意力缺陷多动障碍通常受到遗传和环境因素的影响。虽然近年来已经确定了几个遗传风险因素，但对导致神经行为障碍或导致遗传易感个体进展的环境因素知之甚少。一个引起关注的环境因素是毒死蜱，这是一种广泛用于农业的有机磷农药，在环境中无处不在。在本研究中，我们研究了亚慢性毒死蜱暴露对斑马鱼幼鱼发育过程中焦虑相关行为的影响。我们发现，在发育过程中亚慢性暴露于0.01或0.1 μM毒死蜱可诱导7日龄斑马鱼幼鱼出现特定的行为缺陷。幼虫游泳速度和趋触性下降，但回避行为没有变化。0.001 μM毒死蜱对幼虫的游泳、趋触和回避行为没有影响，1 μM毒死蜱引起幼虫的行为缺陷，但也引起幼虫形态缺陷。由于趋触性，对边缘的偏好，是一种焦虑相关的行为在斑马鱼幼虫，我们建议，亚慢性毒死蜱暴露干扰焦虑相关行为的发展。这项研究的结果提供了一个很好的起点，检查的分子，细胞，发育和神经机制，受环境相关浓度的有机磷农药。更详细地了解这些机制是重要的预测模型和完善的卫生政策，以防止毒物引起的神经行为障碍的发展。
Neurobehavioral disorders such as anxiety, autism, and attention deficit hyperactivity disorders are typically influenced by genetic and environmental factors. Although several genetic risk factors have been identified in recent years, little is known about the environmental factors that either cause neurobehavioral disorders or contribute to their progression in genetically predisposed individuals. One environmental factor that has raised concerns is chlorpyrifos, an organophosphate pesticide that is widely used in agriculture and is found ubiquitously in the environment. In the present study, we examined the effects of sub-chronic chlorpyrifos exposure on anxiety-related behavior during development using zebrafish larvae. We found that sub-chronic exposure to 0.01 or 0.1 μM chlorpyrifos during development induces specific behavioral defects in 7-day-old zebrafish larvae. The larvae displayed decreases in swim speed and thigmotaxis, yet no changes in avoidance behavior were seen. Exposure to 0.001 μM chlorpyrifos did not affect swimming, thigmotaxis, or avoidance behavior and exposure to 1 μM chlorpyrifos induced behavioral defects, but also induced defects in larval morphology. Since thigmotaxis, a preference for the edge, is an anxiety-related behavior in zebrafish larvae, we propose that sub-chronic chlorpyrifos exposure interferes with the development of anxiety-related behaviors. The results of this study provide a good starting point for examination of the molecular, cellular, developmental, and neural mechanisms that are affected by environmentally relevant concentrations of organophosphate pesticides. A more detailed understanding of these mechanisms is important for the development of predictive models and refined health policies to prevent toxicant-induced neurobehavioral disorders.
登录
查看更多内容
影响因子：
1.3
作者：
Colwill, Ruth M.;Creton, Robbert
通讯作者：
Creton, Robbert
影响因子：
2.9
作者：
Braquenier, Jean-Baptiste;Quertemont, Etienne;Plumier, Jean-Christophe
通讯作者：
Plumier, Jean-Christophe
影响因子：
2.9
作者：
Chen J;Huang C;Zheng L;Simonich M;Bai C;Tanguay R;Dong Q
通讯作者：
Dong Q
影响因子：
8
作者：
Bouchard, Maryse F.;Bellinger, David C.;Weisskopf, Marc G.
通讯作者：
Weisskopf, Marc G.
中枢神经系统血清素能和多巴胺能突触活性在产前或新生儿毒性菌暴露后成年。
影响因子：
10.4
作者：
通讯作者：
--