OsPLS4 Is Involved in Cuticular Wax Biosynthesis and Affects Leaf Senescence in Rice
OsPLS4 Is Involved in Cuticular Wax Biosynthesis and Affects Leaf Senescence in Rice
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OsPLS4 参与角质层蜡生物合成并影响水稻叶片衰老
DOI：
10.3389/fpls.2020.00782
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发表时间：
2020-06
影响因子：
5.6
通讯作者：
Haohua He
中科院分区：
文献类型：
--
作者：
Dahu Zhou;Ting Li;Yaolong Yang;Ziyang Qu;Linjuan Ouyang;Zhishu Jiang;Xiaoli Lin;Changlan Zhu;Liyuan Peng;Junru Fu;Xiaosong Peng;Jianmin Bian;Wenbang Tang1;Jie Xu;Haohua He
叶片衰老是影响水稻生长发育和产量的最常见因素之一。虽然已经鉴定了许多影响叶片衰老的基因，但很少有人描述与角质层蜡质合成有关的水稻叶片早衰。在这里，我们克隆并鉴定了水稻早熟叶片衰老4(PLS4)基因，该基因编码脂肪酸生物合成途径中的一个可能的3-氧酰基还原酶。在叶绿体中观察到了OsPLS4的亚细胞定位。OsPLS4中的单核苷酸替换减少了叶表皮蜡质，大多数蜡质生物合成相关基因的表达水平下调。透射电子显微镜显示，pls4突变体的叶绿体发育存在缺陷。进一步研究发现，与对照相比，突变体pls4的叶绿素(Chl)含量降低，光合作用速率降低，导致抽穗期ROS显著积累。这些结果证实了PLS4植物叶片的早衰。低温处理表明，突变体对冷胁迫的敏感性高于野生型。综上所述，上述结果表明，OsPLS4突变影响水稻角质层蜡质的生物合成和叶绿体的发育，导致角质层蜡质减少和叶片早衰。
Leaf senescence is one of the most common factors that affects the growth and yield of rice. Although numerous genes affecting leaf senescence have been identified, few involved in cuticular wax synthesis have been described for rice premature leaf senescence. Here, we cloned and characterized Premature Leaf Senescence 4 (PLS4) in rice (Oryza sativa), which encodes a putative 3-oxoacyl-reductase in the fatty acid biosynthetic pathway. Subcellular localization of OsPLS4 was observed in the chloroplast. A single nucleotide substitution in OsPLS4 reduced leaf cuticular wax, and the expression levels of most wax biosynthesis-associated genes were downregulated. TEM showed chloroplast development were defective in the pls4 mutant. Further investigation revealed that the chlorophyll (Chl) content was reduced in the pls4 mutant compared with the WT and that the photosynthesis rate was lower, which caused ROS dramatic accumulation at the heading stage. These results confirmed premature leaf senescence in pls4 plants. Cold treatment indicated that the mutant was more sensitive than the WT was to cold stress. Together, all the above results indicate that the OsPLS4 mutation affects cuticular wax biosynthesis and chloroplast development in rice, causing reduced cuticular wax and premature leaf senescence.
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影响因子：
5.1
作者：
Yasuhito Sakuraba;Su-Hyun Han;Hyun-Jung Yang;Weilan Piao;N. Paek
通讯作者：
Yasuhito Sakuraba;Su-Hyun Han;Hyun-Jung Yang;Weilan Piao;N. Paek
PGL 编码叶绿素加氧酶 1，影响叶片衰老并间接影响水稻的产量和品质
影响因子：
6.9
作者：
Yang Y;Xu J;Huang L;Leng Y;Dai L;Rao Y;Chen L;Wang Y;Tu Z;Hu J;Ren D;Zhang G;Zhu L;Guo L;Qian Q;Zeng D
通讯作者：
Zeng D
影响因子：
7.2
作者：
Morita, Ryouhei;Sato, Yutaka;Kusaba, Makoto
通讯作者：
Kusaba, Makoto
影响因子：
7.4
作者：
Denise Weidenbach;Marcus Jansen;R. Franke;G. Hensel;W. Weissgerber;Sylvia Ulferts;Irina Jansen;L. Sc
通讯作者：
Denise Weidenbach;Marcus Jansen;R. Franke;G. Hensel;W. Weissgerber;Sylvia Ulferts;Irina Jansen;L. Sc
影响因子：
11.4
作者：
Jiang, Huawu;Chen, Yaping;Wu, Guojiang
通讯作者：
Wu, Guojiang