Involvement of mucosal flora and enterochromaffin cells of the caecum and descending colon in diarrhoea-predominant irritable bowel syndrome.
Involvement of mucosal flora and enterochromaffin cells of the caecum and descending colon in diarrhoea-predominant irritable bowel syndrome.
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腹泻型肠易激综合征中盲肠和降结肠粘膜菌群和肠嗜铬细胞的参与
DOI：
10.1186/s12866-021-02380-2
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发表时间：
2021-11-13
期刊：
影响因子：
4.2
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Yang J;Wang P;Liu T;Lin L;Li L;Kou G;Zhou R;Li P;Li Y
背景越来越多的证据支持肠道菌群在肠易激综合征（IBS）中的关键作用。肠嗜铬 (EC) 细胞合成的血清素受到肠道微生物群的影响，并且据报道与 IBS 存在相互作用。 IBS 中盲肠和结肠粘膜微生物群的比较很少被研究。本研究的目的是调查肠道微生物群、盲肠和降结肠中的 EC 细胞以及腹泻为主的 IBS (IBS-D) 症状之间的关系。 结果共有 22 名 IBS-D 患者和 22 名健康对照 (HC) 纳入我们的研究。 IBS-D 患者的汉密尔顿焦虑 (HAM-A) 和汉密尔顿抑郁 (HAM-D) 等级显着升高。此外，IBS-D患者的排便频率高于健康人。在优势菌属中，IBS-D患者盲肠样本中瘤胃球菌_扭矩_群的相对丰度增加，而拉乌尔氏菌和梭杆菌的相对丰度较低。在降结肠中，IBS-D患者中瘤胃球菌_扭矩_群和Dorea的丰度增加，而梭杆菌的丰度减少。在粘膜相关微生物群方面，降结肠和盲肠之间没有观察到差异。 IBS-D患者盲肠中的EC细胞数量高于HC，并且IBS-D患者的盲肠和降结肠中TPH1的mRNA和蛋白水平表达较高。相关分析显示瘤胃球菌扭矩组与HAM-A、HAM-D、EC细胞数、IBS-SSS、腹痛程度、腹痛频率、排便次数呈正相关。 Dore丰度与EC细胞数、IBS-SSS、HAM-A、HAM-D及腹痛频率呈正相关。结论IBS-D患者EC细胞数增多，TPH1表达高于HC。此外，瘤胃球菌扭矩组和 Dorea 似乎是未来研究治疗 IBS-D 患者的有希望的目标。
BackgroundAccumulating evidence supports the pivotal role of intestinal flora in irritable bowel syndrome (IBS). Serotonin synthesis by enterochromaffin (EC) cells is influenced by the gut microbiota and has been reported to have an interaction with IBS. The comparison between the microbiota of the caecal and colonic mucosa in IBS has rarely been studied. The aim of this study was to investigate the relationship between the gut microbiota, EC cells in caecum and descending colon, and diarrhoea-predominant IBS (IBS-D) symptoms.ResultsA total of 22 IBS-D patients and 22 healthy controls (HCs) were enrolled in our study. Hamilton anxiety (HAM-A) and Hamilton depression (HAM-D) grades increased significantly in IBS-D patients. In addition, the frequency of defecation in IBS-D patients was higher than that in HCs. Among the preponderant bacterial genera, the relative abundance of theRuminococcus_torques_ groupincreased in IBS-D patients in caecum samples whileRaoultellaandFusobacteriumwere less abundant. In the descending colon, the abundance of theRuminococcus_torques_groupandDoreaincreased in IBS-D patients andFusobacteriumdecreased. No difference was observed between the descending colon and caecum in regards to the mucosal-associated microbiota. The number of EC cells in the caecum of IBS-D patients was higher than in HCs and the expression of TPH1 was higher in IBS-D patients both in the caecum and in the descending colon both at the mRNA and protein level. Correlation analysis showed that theRuminococcus_torques_groupwas positively associated with HAM-A, HAM-D, EC cell number, IBS-SSS, degree of abdominal pain, frequency of abdominal pain and frequency of defecation. The abundance ofDoreawas positively associated with EC cell number, IBS-SSS, HAM-A, HAM-D and frequency of abdominal pain.ConclusionsEC cell numbers increased in IBS-D patients and the expression of TPH1 was higher than in HCs. TheRuminococcus torques groupandDoreafurthermore seem like promising targets for future research into the treatment of IBS-D patients.
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影响因子：
4.3
作者：
Lee, Kang Nyeong;Lee, Oh Young
通讯作者：
Lee, Oh Young
青春双歧杆菌对慢性应激后焦虑和抑郁样表型的预防作用：肠道菌群-炎症轴的作用
影响因子：
3
作者：
Guo, Ying;Xie, Jian-Ping;Luo, Huai-Rong
通讯作者：
Luo, Huai-Rong
影响因子：
7.6
作者：
Kajander, K.;Myllyluoma, E.;Korpela, R.
通讯作者：
Korpela, R.
DOI：
10.1152/ajpgi.00044.2015
发表时间：
2016-03-15
影响因子：
4.5
作者：
Ringel-Kulka, Tamar;Benson, Andrew K.;Ringel, Yehuda
通讯作者：
Ringel, Yehuda
影响因子：
4.3
作者：
Malinen, Erja;Krogius-Kurikka, Lotta;Palva, Airi
通讯作者：
Palva, Airi